Hiroki Watanabe
National Institutes of Natural Sciences, Japan
36 Papers
93 Citations
Hiroki Watanabe is an academic researcher from National Institutes of Natural Sciences, Japan. The author has contributed to research in topics: Chemistry & Cellulose. The author has an hindex of 15, co-authored 35 publications. Previous affiliations of Hiroki Watanabe include Kanazawa University & Nagoya University.
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Papers
Trade-off between processivity and hydrolytic velocity of cellobiohydrolases at the surface of crystalline cellulose.
Akihiko Nakamura,Hiroki Watanabe,Takuya Ishida,Takayuki Uchihashi,Masahisa Wada,Masahisa Wada,Toshio Ando,Kiyohiko Igarashi,Masahiro Samejima +8 more
TL;DR: It is found that high moving velocity of enzyme molecules on the surface is associated with a high dissociation rate constant from the substrate, which means weak interaction between enzyme and substrate, and indicates that there is a trade-off between processivity and hydrolytic velocity among processive cellulases.
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Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps.
Keiichi Inoue,Satoshi P. Tsunoda,Satoshi P. Tsunoda,Manish Singh,Sahoko Tomida,Shoko Hososhima,Masae Konno,Ryoko Nakamura,Hiroki Watanabe,Paul-Adrian Bulzu,Horia L. Banciu,Adrian-Ştefan Andrei,Takayuki Uchihashi,Rohit Ghai,Oded Béjà,Hideki Kandori +15 more
TL;DR: The function, trimeric structure, and H+ transport mechanism of SzR are similar to that of xenorhodopsin (XeR), a light-driven inward H+ pumping microbial rhodopsins, implying that they evolved convergently.
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Dynamic structural states of ClpB involved in its disaggregation function.
Takayuki Uchihashi,Yo-hei Watanabe,Yosuke Nakazaki,Takashi Yamasaki,Hiroki Watanabe,Takahiro Maruno,Kentaro Ishii,Susumu Uchiyama,Susumu Uchiyama,Chihong Song,Kazuyoshi Murata,Ryota Iino,Ryota Iino,Toshio Ando +13 more
TL;DR: The HS-AFM movies demonstrate massive conformational changes of the hexameric ring during ATP hydrolysis, from a round ring to a spiral and even to a pair of twisted half-spirals, providing a comprehensive view for the ATP-driven oligomeric-state transitions that enable ClpB to disentangle protein aggregates.
Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy
Daisuke Yamamoto,Azuma Taoka,Takayuki Uchihashi,Hideaki Sasaki,Hiroki Watanabe,Toshio Ando,Yoshihiro Fukumori +6 more
TL;DR: Findings suggest that magnetosomes are covered with MamA oligomers in near-native environments and provide important clues to the supramolecular architecture of the bacterial organelle, the magnetosome, and insight into the function of the proteins localized in the organelle.
Desiccation-induced fibrous condensation of CAHS protein from an anhydrobiotic tardigrade.
Maho Yagi-Utsumi,Maho Yagi-Utsumi,Kazuhiro Aoki,Hiroki Watanabe,Chihong Song,Seiji Nishimura,Tadashi Satoh,Saeko Yanaka,Saeko Yanaka,Christian Ganser,Sae Tanaka,Sae Tanaka,Vincent Schnapka,Vincent Schnapka,Ean Wai Goh,Yuji Furutani,Yuji Furutani,Kazuyoshi Murata,Takayuki Uchihashi,Takayuki Uchihashi,Kazuharu Arakawa,Koichi Kato,Koichi Kato +22 more
TL;DR: In this article, the reversible self-assembling property of CAHS1 proteins was investigated in extremotolerant tardigrades, and it was found that CAHS proteins homo-oligomerized via the C-terminal α-helical region and formed a hydrogel as their concentration increased.
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