Isolation and characterization of extremely minor gangliosides, GM1b and GD1 alpha, in adult bovine brains as developmentally regulated antigens.

http://iopscience.iop.org/article/10.1088/1468-6996/12/2/023001/pdf

Soft particle analysis of electrokinetics of biological cells and their model systems

https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2897%2901386-0

Preparation of peptides which mimic glycosphingolipids by using phage peptide library and their modulation on β‐galactosidase activity

Isolation and characterization of ganglioside GM1b from normal human brain.

Ganglioside GM1b as an influenza virus receptor

Studies on developmental changes of ganglioside synthesis and compositions were carried out using rat bone marrow cells, spleen and thymus. Ganglioside synthesis was studied by assaying sialyltransferase for GM3 synthesis and GM1b synthesis. In bone marrow cells, peaks of both enzyme activities occurred coincidentally in 2- to 5-week-old rats. In spleen, the highest activities of these enzymes were observed in one-week-old rats. GM1b synthesis in the thymus was almost constant after birth, but GM3 synthesis could not be detected at any age examined. Developmental changes of gangliosides in these tissues were analyzed by thin layer chromatography. Gangliosides corresponding to GM1b and GM3 were recognized in each tissue. The ganglioside content of the bone marrow cells increased in 2- to 5-week-old rats. Ganglioside corresponding to GM1b was isolated from the bone marrow cells, and its structure was confirmed to be the same as that of GM1b by sequential hydrolysis of the ganglioside with glycosidases. GM3 was a predominant ganglioside in newborn rat spleen. Ganglioside content in the spleen increased during 2-5 weeks after birth and became constant after 9 weeks. In the thymus, more than 10 different gangliosides were discriminated, but significant changes of ganglioside pattern with the progress of development could not be observed. The developmental change of the ganglioside composition coincided well with the change of sialyltransferase activities.

Developmental Changes of Ganglioside Compositions and Biosyntheses in Rat Bone Marrow Cells, Spleen and Thymus

Flow cytometric analysis using anti-glycolipid antiserum was used on rat bone marrow cells to determine the relation between the glycolipid species expressed on cell surfaces and cell differentiation. Four kinds of antibodies against gangliotriaosylceramide (Gg3Cer), gangliotetraosylceramide (Gg4Cer), fucogangliotetraosylceramide (IV2 alpha Fuc-Gg4Cer) and IV3 alpha Gal-fucogangliotetraosylceramide (IV3 alpha GalIV2 alpha Fuc-Gg4Cer, blood group B lipid) were used. The cells sorted out by each anti-glycolipid antiserum were stained with May-Grunwald-Giemsa reagent and identified by microscopy. In the erythropoietic group, only polychromatic erythroblasts had these four glycolipids on their cell surfaces; none appeared on differentiated erythrocytes. These glycolipids were expressed during the early stages of immature granulocytes, especially in the promyelocyte and myelocyte stages of eosinophilic and neutrophilic granulocytes. Very limited populations of lymphocytes were sorted out as asialoganglioside-expressing cells. We concluded that asialogangliosides are useful differentiation markers for the erythropoietic and granulopoietic cells of rat bone marrow, and that anti-asialoganglioside antibody-flow cytometry is a very useful technique with which to isolate immature granulocytes and erythropoietic cells from rat bone marrow cells.

Asialogangliosides as differentiation markers of rat erythropoietic and granulopoietic cells in rat bone marrow. Flow cytometric analysis of rat bone marrow cells using anti-asialoganglioside antibodies.

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Hirohisa Kimura

Author Tools

Chat about Author

Papers

Developmental Changes of Ganglioside Compositions and Biosyntheses in Rat Bone Marrow Cells, Spleen and Thymus

Asialogangliosides as differentiation markers of rat erythropoietic and granulopoietic cells in rat bone marrow. Flow cytometric analysis of rat bone marrow cells using anti-asialoganglioside antibodies.