Hirofumi Doi
University of Tokyo
21 Papers
50 Citations
Hirofumi Doi is an academic researcher from University of Tokyo. The author has contributed to research in topics: Streptavidin & Chemistry. The author has an hindex of 8, co-authored 21 publications. Previous affiliations of Hirofumi Doi include Tokyo Metropolitan University.
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Papers
Transgenic overexpression of USP15 in the heart induces cardiac remodeling in mice.
Yoshitaka Isumi,Tsuyoshi Hirata,Hiroshi Saitoh,Tomoya Miyakawa,Kenji Murakami,Gen Kudoh,Hirofumi Doi,Kohtaro Ishibashi,Hiroto Nakajima +8 more
TL;DR: Results indicate that USP15 is involved in the regulation of hypertrophic responses in cardiac muscle through transcriptional and post-translational modulation of SLIM1.
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Epiregulin Recognition Mechanisms by Anti-epiregulin Antibody 9E5: STRUCTURAL, FUNCTIONAL, AND MOLECULAR DYNAMICS SIMULATION ANALYSES
Yuji Kado,Eiichi Mizohata,Satoru Nagatoishi,Mariko Iijima,Keiko Shinoda,Takamitsu Miyafusa,Taisuke Nakayama,Takuma Yoshizumi,Akira Sugiyama,Takeshi Kawamura,Young-Hun Lee,Hiroyoshi Matsumura,Hirofumi Doi,Hideaki Fujitani,Tatsuhiko Kodama,Yoshikazu Shibasaki,Kouhei Tsumoto,Tsuyoshi Inoue +17 more
TL;DR: The superimposed structure of 9E5(Fab)·EPR on the known complex structure of EGF·EGFR showed that the 9E 5( Fab) paratope overlaps with Domains I and III on the EGFR, which reveals that the EPR complex could not bind to the EGfr.
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Crystal structure of streptavidin mutant with low immunogenicity
Tatsuya Kawato,Eiichi Mizohata,Tomohiro Meshizuka,Hirofumi Doi,Takeshi Kawamura,Hiroyoshi Matsumura,Kyohei Yumura,Kouhei Tsumoto,Tatsuhiko Kodama,Tsuyoshi Inoue,Akira Sugiyama +10 more
TL;DR: High-resolution X-ray structural analyses of LISA-314 and wild-type streptavidin and the effect of substitutions on the protein function and the three-dimensional structure demonstrated the LISA
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Construction and characterization of functional anti-epiregulin humanized monoclonal antibodies.
Young-Hun Lee,Mariko Iijima,Yuji Kado,Eiichi Mizohata,Tsuyoshi Inoue,Akira Sugiyama,Hirofumi Doi,Yoshikazu Shibasaki,Tatsuhiko Kodama +8 more
TL;DR: The data support that HM1 exerts potent antibody-dependent cellular cytotoxicity (ADCC) and may have potential for further development as a candidate therapeutic agent and research tool.
15
Structure-based design of a streptavidin mutant specific for an artificial biotin analogue
Tatsuya Kawato,Eiichi Mizohata,Yohei Shimizu,Tomohiro Meshizuka,Tomohiro Yamamoto,Noriaki Takasu,Masahiro Matsuoka,Hiroyoshi Matsumura,Tatsuhiko Kodama,Motomu Kanai,Hirofumi Doi,Tsuyoshi Inoue,Akira Sugiyama +12 more
TL;DR: This study improves the binding pocket of LISA-314 to abolish its affinity for endogenous BTN species, therefore ensuring that the newly designed LISA-314 binds only artificial BTN analogue.
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