6 Papers
15 Citations
Heng Wu is an academic researcher from University of Texas Southwestern Medical Center. The author has contributed to research in topics: Actin cytoskeleton & Cytoskeleton. The author has an hindex of 4, co-authored 6 publications.
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Papers
Mical links semaphorins to F-actin disassembly
Ruei-Jiun Hung,Umar Yazdani,Jimok Yoon,Heng Wu,Taehong Yang,Nidhi Gupta,Zhiyu Huang,Willem J. H. van Berkel,Jonathan R. Terman +8 more
TL;DR: Mical is a novel F-actin-disassembly factor that provides a molecular conduit through which actin reorganization—a hallmark of cell morphological changes including axon navigation—can be precisely achieved spatiotemporally in response to semaphorins.
A simple and efficient method for generating high-quality recombinant Mical enzyme for in vitro assays.
TL;DR: A new purification strategy that enables the rapid and efficient purification of milligram quantities of highly-pure and >99% active Mical protein will aid research objectives designed to characterize the biochemical, enzymology, and structural biology of Mical and its effects on actin filament dynamics.
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Enhanced Production of the Mical Redox Domain for Enzymology and F-actin Disassembly Assays.
TL;DR: In this article, a family of unusual actin regulators, the MICALs, which are flavoprotein monooxygenase/hydroxylase enzymes that associate with FAD and use the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) in Redox reactions, are identified.
4
Data presenting a modified bacterial expression vector for expressing and purifying Nus solubility-tagged proteins.
TL;DR: The data herein present a modified bacterial expression vector useful for expressing proteins fused to the Nus solubility tag and separating such target proteins from the NUs tag during protein purification.
4
The MICALs are a Family of F-actin Dismantling Oxidoreductases Conserved from Drosophila to Humans.
TL;DR: The MICALs are defined as an important phylogenetically-conserved family of catalytically-acting F-actin disassembly factors that drive F- actin dis assembly in vivo, reshaping cells and their membranous extensions.