Novel crosslinked chitosan for enhanced adsorption of hexavalent chromium in acidic solution

Immobilization of Lipases – A Review. Part I: Enzyme Immobilization

Influencing factors on single-cell protein production by submerged fermentation: A review

Effective removal of sulfur dyes from water by biosorption and subsequent immobilized laccase degradation on crosslinked chitosan beads

Two-dimensional layered nanomaterial Ti3C2TX (a member of the MXene family) was used to immobilise enzyme sarcosine oxidase to fabricate a nanostructured biosensor. The device was applied for detection of sarcosine, a potential prostate cancer biomarker, in urine for the first time. The morphology and structures of MXene have been characterised by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Electrochemical measurements, SEM and AFM analysis revealed that MXene interfaced with chitosan is an excellent support for enzyme immobilisation to fabricate a sensitive biosensor exhibiting a low detection limit of 18 nM and a linear range up to 7.8 µM. The proposed biosensing method also provides a short response time of 2 s and high recovery index of 102.6% for detection of sarcosine spiked into urine sample in a clinically relevant range.

/pdf/ultrasensitive-ti3c2tx-mxene-chitosan-nanocomposite-based-9r96kgbp0h.pdf

Ultrasensitive Ti3C2TX MXene/Chitosan Nanocomposite-Based Amperometric Biosensor for Detection of Potential Prostate Cancer Marker in Urine Samples

The effect of glutaraldehyde solution concentration, cross-linking time, cross-linking pH and cross- linking temperature on the enzyme activity of the immobilized β-galactosidase on Chitosan beads were studied. The enzyme activity was measured after immobilized by detecting the absorbance value at 420 nm.The results were as follows: the immobilized enzyme activity reached the maximum when the concentration of glutaraldehyde solution was 0.3%.The immobilized enzyme had optimal cross-linking time of 3h, optimal cross-linking pH of 6.5, optimal cross-linking temperature of 25°C, under these conditions, the immobilized enzyme activity reached 101, 96, 90 U/g, respectively.

The Effect of Glutaraldehyde Cross-Linking on the Enzyme Activity of Immobilized &beta-Galactosidase on Chitosan Bead

Using Yam starch as raw material, the fermentation technology of Single Cell Protein (SCP) was optimized through by active dry yeast fermentation through single factor and orthogonal test in this study. The optimal conditions were showed as followed: 30 mL the medium volume in 250 mL flask, inoculum size17%, fermentation time 69 h, pH 5.0. Under these conditions, the production of SCP reached 241.54±0.15 (g wet weight/100 g Dry starch.

Optimization of Fermentation Technology for Producing Single Cell Protein from Yam Starch by Orthogonal Test

Response Surface Methodology (RSM) was advantageously used to optimize production conditions of immobilization of β-galactosidase onto chitosan beads. The influence of immobilization conditions on the immobilized enzyme activity was studied by using Box-Behnken experimental design, resulting in the appropriate average values of OD420 and the maximum value of immobilized enzyme activity. This illustrated that the experimental model was reliable and the experimental results were of good stability. Analysis of variance was performed to determine the adequacy and significance of the quadratic model. Various model parameters also could be seen from F value that the interaction between three factors was not particularly obvious, but effects of X 1, X1 2 , X2 2 and X3 2 on the activity of immobilized enzyme were very obvious. The optimisation parameters studied in accordance with the results were: the appropriate average values of OD 420 loaded in the range from 0.713 to 0.721. Furthermore the immobilized enzyme activity reached about 162U/g when the adsorption time, adsorption pH and cross-linking pH were 4h, 7 and 7, respectively. Thus, the relative activity of β-galactosidase immobilized increased more than 37% compared with the previous single factor research.

Optimisation of Immobilisation Conditions of β-galactosidase onto Chitosan Beads Using Response Surface Methodology

This present study is based on the single factor test and the significant factors of emulsification conditions, which influence the process of microencapsulation of B. bifidum BB28 and BB01, will be screen out by Plackett-Burman experimental. Bifidobacterium BB0I and BB28 were encapsulated by using emulsion method respectively. Several factors, such as alginate concentration, cell suspension-alginate ratios, CaCl2 concentration, Tween 80 concentration, emulsification time, encapsulating time and oil-water ratios have been investigated, the results showed that sodium alginate concentration, cell suspension-alginate ratios, encapsulating time and emulsification time have a significant effect for Bifidobacterium BB01 microcapsules; CaCl2 concentration, cell suspension-alginate ratios, sodium alginate concentration and emulsification time have a significant effect for Bifidobacterium BB28 microcapsules.

Screening of Emulsification Conditions on Microcapsulation of B. bifidum BB01 and BB28 by Plackett-burman Design

The invention discloses a lactic acid bacteria formula goat milk powder and a preparation method thereof. The lactic acid bacteria formula goat milk powder comprises the following components in partsby mass: 1000 parts of fresh goat's milk, 2.5 to 5 parts of stachyose, 4 to 7.5 parts of sodium glutamate, 1 to 3 parts of xylooligosaccharide, 1 to 3 parts of trehalose, 2 to 4 parts of taurine, 0.3to 1 part of vitamin C, 0.2 to 0.5 parts of lactobacillus bulgaricus powder and 0.2 to 0.5 parts of Streptococcus thermophilus powder. The pretreated fresh goat milk is added with the relevant additive to form a mixture; the mixture is divided into two parts, and one part is processed to form concentrated milk for standby; another one is added with the lactobacillus bulgaricus powder and Streptococcus thermophilus powder for culture, and then concentrated milk and other additives are added, and lactic acid bacteria goat milk powder is obtained by spray drying. The lactic acid bacteria formulagoat milk powder can promote the growth of lactobacillus bulgaricus and Streptococcus thermophilus in goat milk, can also provide protection to both bacteria during spray drying, and improves the survival rate. The lactic acid bacteria formula goat milk powder can also promote the proliferation of intestinal probiotics and the absorption of minerals, increases intestinal peristalsis, prevents constipation and other functions, and benefits consumers' health.

Lactic acid bacteria formula goat milk powder and preparation method thereof

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