Hazel E. Jenkins
University of Dundee
9 Papers
474 Citations
Hazel E. Jenkins is an academic researcher from University of Dundee. The author has contributed to research in topics: Lamin & Nuclear matrix. The author has an hindex of 8, co-authored 9 publications.
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Papers
Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs.
TL;DR: The results indicate that both the recruitment and maintenance of Nup153 at the pore are dependent upon the integrity of the lamina, and the distribution of other nucleoporins is unaffected.
158
GST-lamin fusion proteins act as dominant negative mutants in Xenopus egg extract and reveal the function of the lamina in DNA replication
TL;DR: The results are consistent with the hypothesis that lamina assembly precedes and is required for the formation of replication centers but does not support those centers directly.
131
•Journal Article
Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.
TL;DR: Immunoblotting analysis of isolated nuclei and nuclear matrix fractions indicated that lamin-depleted nuclei still accumulated nucleoporins and PCNA, indicating that these nuclei do assemble a nuclear matrix.
112
Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: evidence from cell-free egg extracts
Martin W. Goldberg,Hazel E. Jenkins,Terry Allen,William G.F. Whitfield,Christopher J. Hutchison +4 more
TL;DR: Xenopus egg extracts which assemble replication competent nuclei in vitro were depleted of lamin B3 using monoclonal antibody L6 5D5 linked to paramagnetic beads and failed to initiate semi-conservative DNA replication, it seems likely that the inability of lamination-depleted extracts to assemble a replication competent nucleus is a direct consequence of a failure to assembling a lamina.