Soybean-source oleosin gene seed specificity promoter and application thereof

The invention relates to the technical field of biology, in particular to a seed-specific promoter sequence separated from soybean and applications thereof The nucleotide sequence is shown in SEQ No1 The seed-specific promoter sequence is used as the seed-specific promoter of soybean In the invention, the stearic acid-acyl carrier protein (ACP) desaturase promoter of soybean is cloned and the tissue expression characteristics and expression efficiency of the promoter in soybean are researched, thus an evidence for the effective use of the promoter is provided; and the promoter provides a good tool for the transgenic research of soybean and creates conditions for the researches and developments by utilizing the soybean seed as bioreactor

Seed-specific promoter separated from soybean and applications thereof

Tangerine chlorenchyma specific promoter

The invention discloses a method of improving the soybean genetic transformation efficiency by virtue of the synergism of a surfactant and ultrasonic waves and an application of the method. The method is an agrobacterium tumefaciens mediated soybean cotyledonary node genetic transformation method and comprises the following steps: infecting a soybean cotyledonary node explant by virtue of a recombinant agrobacterium tumefaciens containing a target DNA (Deoxyribonucleic Acid) to obtain an infected explant, co-culturing the infected explant to obtain the infected explant after co-culture, and completing the transformation, wherein the infected explant obtained by infecting the soybean cotyledonary node explant by virtue of the recombinant agrobacterium tumefaciens containing the target DNA is the infected explant obtained by arranging the soybean cotyledonary node explant into an infection liquid of the recombinant agrobacterium tumefaciens to carry out ultrasonic treatment, the infection liquid contains the surfactant, and scratches are not made at the cotyledonary node position of the soybean cotyledonary node explant. The method can be used for culturing a novel variety of transgenic soybeans as well as excavating and identifying gene functions.

Method of improving soybean genetic transformation efficiency by virtue of synergism of surfactant and ultrasonic waves and application of method

The invention relates to a specific promoter in a leguminous plant legume related tissue and application thereof. The promoter is particularly suitable for driving the specific expression of exogenous genes in the leguminous plant legume related tissue, such as legumes or seed leaves, and the expression level of the exogenous genes in other tissues of leguminous plants is extremely low or the exogenous genes are not expressed in the other tissues of the leguminous plants. The expression specificity and specificity of the promoter are very high. The invention further provides constructions, expression kits, carriers and the like with the promoter. For transgenic leguminous plants containing the promoter disclosed by the invention, agronomic traits of the leguminous plants can be specifically improved, so that the influence on the other tissues of the leguminous plants caused by the introduction of the exogenous genes is effectively avoided, the exogenous genes can be specifically expressed in the legume related tissue, and thus, various related products are obtained.

Specific promoter in leguminous plant legume related tissue and application thereof

The invention relates to a specific promoter of soybean seeds, which belongs to the sequence and use of a soybean aspartic protease gene promoter. The nucleotide sequence of the promoter is represented by the sequence No.1. The preparation method of the promoter comprises the following steps of: cloning the upstream distal sequence of gene soyAP1, analyzing the upstream sequence of the ATG of the gene soyAP1 and determining the deleted fragments SAP1, SAP2, soyAP1-p and SAP4; and amplifying the soyAP1-p fragment, linking the amplified fragment of the soyAP1-p with a cloning vector pMD 18-T, performing identification, measuring the sequence, and verifying the sequence. In the invention, the soyAP1-p promoter of soybean is cloned and expressed in the soybean seeds specifically, and the promoter obtained can be used as a favorable tool in researches on soybean transgenosis and particularly creates conditions for researching and developing soybean seed bioreactors.

Specific promoter of soybean seeds and use thereof

The invention relates to a high-efficiency genetic transformation method using a soybean Jilin No.35 embryonic tip as an explant and belongs to the technical field of plant transgenosis. The method comprises the following steps of: selecting Jilin No.35 soybean seeds with integrated seed coat and without diseases; performing ultrasonic treatment on the explant; transferring the infected embryonic tip explant into a co-culture medium; co-cultivating; inoculating into a rooting medium; and transplanting to a nutrition pot after the root grows strong. According to the method, the regeneration cycle is shortened. Through the adoption of a strong and effect infection method, browning degree and necrosis degree during co-cultivation are reduced, and conversion rate is increased.

High-efficiency genetic transformation method using soybean Jilin No.35 embryonic tip as explant

The invention relates to an application of a soybean MYB transcription factor gene in improvement of soybean isoflavone biosynthesis and belongs to the technical field of genetic engineering. The GmMYB9 gene is 1188bp and encodes 395 amino acids; a yeast one-hybrid experiment result indicates that GmMYB9 has transcriptional activation activity, and a subcellular localization result proves that GmMYB9 is located in a cell nucleus; a real-time fluorescence quantification PCR (polymerase chain reaction) result shows that expression tendency of GmMYB9 in a soybean development process from immature embryos to mature seeds is consistent with accumulation tendency of isoflavone, and the expression quantity of GmMYB9 in a variety with high isoflavone content is higher than that of GmMYB9 in a variety with low isoflavone content; the isoflavone content of seeds and leaves of T2-generation transgenic soybeans with the transformed GmMYB9 gene is increased, therefore, the GmMYB9 gene participates in regulation of the soybean isoflavone biosynthesis and has great significance in cultivation of the soybean varieties with high isoflavone content.

Application of soybean MYB transcription factor gene in improvement of soybean isoflavone biosynthesis

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Papers

Specific promoter of soybean seeds and use thereof

Seed-specific promoter separated from soybean and applications thereof

High-efficiency genetic transformation method using soybean Jilin No.35 embryonic tip as explant

Application of soybean MYB transcription factor gene in improvement of soybean isoflavone biosynthesis