Hans Amesz
Utrecht University
13 Papers
583 Citations
Hans Amesz is an academic researcher from Utrecht University. The author has contributed to research in topics: Initiation factor & Ternary complex. The author has an hindex of 11, co-authored 13 publications.
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Papers
Purification and Characterization of a Protein Factor that Reverses the Inhibition of Protein Synthesis by the Heme‐Regulated Translational Inhibitor in Rabbit Reticulocyte Lysates
TL;DR: A supernatant factor is purified and partially characterized which reverses the effect of the heme-regulated translational inhibitor on protein synthesis in rabbit reticulocyte lysates and restores protein synthesis activity in heme deficient lysate to the level observed in the presence of heme.
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Monoclonal antibodies directed against the cell-surface-exposed part of PhoE pore protein of the Escherichia coli K-12 outer membrane.
TL;DR: It is concluded that the six monoclonal antibodies recognize at least three distinct cell-surface-exposed epitopes whose specificity is determined by the carboxy-terminal 256 amino acids of PhoE protein.
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A Comparison of the Initiation Factors of Eukaryotic Protein Synthesis from Ribosomes and from the Postribosomal Supernatant
TL;DR: A purification procedure is described for the initiation factors of protein synthesis from rabbit reticulocytes from the ribosomal wash and from the postribosomal supernantant, finding no significant difference in specific activity when factors from both sources were compared.
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Regulation of protein synthesis in eukaryotes. Mode of action of eRF, an eIF-2-recycling factor from rabbit reticulocytes involved in GDP/GTP exchange.
TL;DR: The model stresses the catalytic use of eIF-2 in initiation provided that conditions are met for GDP/GTP exchange by a transient complex formation between eif-2 and eRF, and it is shown that phosphorylation of e IF-2 by the hemin-regulated inhibitor (HRI) abolishes the recycling of eIFE-2.
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Initiation of Protein Synthesis in Eukaryotes
TL;DR: The enhanced phosphorylation of the messenger binding factors results not only in an overall stimulation of translation, but especially weak messengers are positively discriminated.
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