Hajime Yaegashi
National Agriculture and Food Research Organization
28 Papers
82 Citations
Hajime Yaegashi is an academic researcher from National Agriculture and Food Research Organization. The author has contributed to research in topics: Rosellinia necatrix & Mycovirus. The author has an hindex of 13, co-authored 26 publications. Previous affiliations of Hajime Yaegashi include Iwate University.
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Papers
Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes
Aki Igarashi,Kousuke Yamagata,Tomokazu Sugai,Yukari Takahashi,Emiko Sugawara,Akihiro Tamura,Hajime Yaegashi,Noriko Yamagishi,Tsubasa Takahashi,Masamichi Isogai,Hideki Takahashi,Nobuyuki Yoshikawa +11 more
TL;DR: As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species.
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Molecular characterization of a new hypovirus infecting a phytopathogenic fungus, Valsa ceratosperma.
TL;DR: Molecular characteristics suggest that this dsRNA represents a new hypovirus that is tentatively designate Valsa ceratospermahypovirus 1 (VcHV1), indicating the existence of two lineages in the family Hypoviridae.
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Characterization of virus-induced gene silencing in tobacco plants infected with apple latent spherical virus
Hajime Yaegashi,T. Yamatsuta,Tsuyoshi Takahashi,Caiyu Li,Masamichi Isogai,T. Kobori,Satoshi T. Ohki,Nobuyuki Yoshikawa +7 more
TL;DR: GFP leaf patch tests using Nicotiana benthamiana line 16c showed that Vp20, one of the three capsid proteins, is a silencing suppressor which interferes with systemic silencing.
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Combinations of two amino acids (Ala40 and Phe75 or Ser40 and Tyr75) in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity.
TL;DR: Site-directed mutagenesis and an agroinoculation assay indicated that the substitution of a single amino acid resulted in extreme reduction in the accumulation of viral genomic RNA, double-stranded RNAs and viral proteins in infiltrated tissues, suggesting that the combinations of the two amino acids at positions 40 and 75 are important for effective replication in host plant cells.
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Apple necrotic mosaic virus, a novel ilarvirus from mosaic-diseased apple trees in Japan and China
Hiroki Noda,Noriko Yamagishi,Hajime Yaegashi,Fei Xing,Jipeng Xie,Shifang Li,Tao Zhou,Tsutae Ito,Nobuyuki Yoshikawa +8 more
TL;DR: Next-generation sequencing analysis of an apple tree showing mosaic symptoms revealed that the tree was infected with three apple latent viruses and a novel ilarvirus that is closely related to Prunus necrotic ringspot virus (PNRSV) and ApMV.
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