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Veterinary Reproduction And Obstetrics

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Clinical Avian Medicine and Surgery

The prime objective of any mutation breeding programme(s) is to develop varieties that would be high yielding coupled with short stature, early maturing and disease resistant. Selection of efficient and effective mutagen(s) is the first priority to produce a high frequency of desirable mutations in mutation breeding programme(s). Variability already present in nature has been the basis of crop improvement through selection and or/recombination approaches but mutation breeding is relatively a quicker method for the improvement of crops (Kharkwal et al., 2004). Induced mutations are the plant breeder’s hope for freedom from complete dependence on nature as only source of genetic variants necessary in plant improvement (Hayes et al., 1955).

Mutagenic Effects of Gamma Rays and EMS in M1 and M2 Generations in Rice (Oryza sativa L.)

The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246). According to the results, we found that treating with kp-10 for 24 h could increase P4 level, the mRNA expression of the steroidogenesis-related gene steroidogenic acute regulatory protein (StAR), free cholesterol content, and decrease miR-1246 expression in BGCs. Overexpression of miR-1246 significantly inhibited P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs, whereas underexpression of miR-1246 significantly reversed this effect in BGCs. Additionally, overexpression of miR-1246 counteracted the accelerative effect of kp-10 on P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs. Conversely, underexpression of miR-1246 enhanced the accelerative effect of kp-10 on P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs. Meanwhile, results of dual-luciferase reporter assays indicated that miR-1246 targeted the 3′UTR of StAR in BGCs. These results demonstrated that kp-10 induced P4 synthesis in BGCs by promoting free cholesterol transport via regulating expression of miR-1246/StAR.

https://www.mdpi.com/2073-4425/13/2/298/pdf?version=1643883659

Kisspeptin-10 Promotes Progesterone Synthesis in Bovine Ovarian Granulosa Cells via Downregulation of microRNA-1246

Expression of kisspeptin and its receptor in different functional classes of ovarian follicle in the buffalo (Bubalus bubalis).

Expression and functional role of kisspeptin and its receptor in the cyclic corpus luteum of buffalo (Bubalus bubalis)

Variability induced for quantitative characters (days to 50% flowering, days to maturity, plant height, panicle length, panicle bearing tillers per plant and grain yield per plant.) were assessed on individual plant basis in M 1 generation. The maximum frequency of chlorophyll mutations per 100M2 plants obtain in M2 generation in 20kR gamma rays + EMS (0.2%) in Kalanamak following by Badshah Bhog, respectively. The Frequency and spectrum of viable mutations per 100M 2 plant maximum in 40kR gamma rays+ EMS (0.2%) in Badshah Bhog genotypes were observed in M 2 generation. Among chlorophyll mutations, Albino mutant was most frequent in both the genotypes. The different types of chlorophyll mutations observed in M 2 generation were Albino, Xantha, Striata and Viridis.

Study of induced polygenic variability in m 1 and chlorophyll mutations in m 2 generations in aromatic rice

Clitoria ternatea L., commonly known as Asian pigeon wings, bluebell vine, blue pea, butterfly pea, cordofan pea and Darwin pea, is a plant species belonging to the Fabaceae family. It is a perennial plant, with elliptic, obtuse leaves. It grows as a vine or creeper, doing well in moist, neutral soil. The most striking feature about this plant is the color of its flowers, a vivid deep blue; solitary, with light yellow markings. Medicinal plants and herbs are increasingly used all over the world for health purpose. All plant parts including Leaves, seeds, bark, fruits, sprouts and stems are used medicinally. The rich biodiversity of our country has provided us plenty of raw materials, yet sustainable linkage must be developed between cultivation, collection and their use Their therapeutic, biological, pharmacological importance and safety is quite useful in making drugs and medicines.

/pdf/clitoria-ternatea-l-a-miraculous-plant-5b8t26cy5c.pdf

Clitoria ternatea L. A Miraculous Plant

The developmental defects of the tubular genital tract including ovaries, oviduct, uterus, cervix and vagina have been reported in cattle as well as buffalo. These defects cause infertility and even complete sterility. As such less information on these aspects in buffalo is available. The present study reported the morphological and histological diagnosis of two rare congenital abnormalities of the uterus i.e. uterus unicornis and uterus didelphys in riverine buffalo.

Congenital anomalies of the uterus in riverine buffalo (Bubalus bubalis)

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