Gwynn Pardee
Novartis
8 Papers
1 Citations
Gwynn Pardee is an academic researcher from Novartis. The author has contributed to research in topics: Biology & Chemistry. The author has an hindex of 5, co-authored 6 publications.
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Papers
Selective VPS34 inhibitor blocks autophagy and uncovers a role for NCOA4 in ferritin degradation and iron homeostasis in vivo
William E. Dowdle,Beat Nyfeler,Jane Nagel,Robert Elling,Shanming Liu,Ellen Triantafellow,Suchithra Menon,Zuncai Wang,Ayako Honda,Gwynn Pardee,John Cantwell,Catherine Luu,Ivan Cornella-Taracido,Edmund Harrington,Peter Fekkes,Hong Lei,Qing Fang,Mary Ellen Digan,Debra Burdick,Andrew F. Powers,Stephen B. Helliwell,Simon D’Aquin,Julie Bastien,Henry Wang,Dmitri Wiederschain,Jenny Kuerth,Philip Bergman,David Schwalb,Jason R. Thomas,Savuth Ugwonali,Fred Harbinski,John A. Tallarico,Christine D. Wilson,Vic E. Myer,Jeffery A. Porter,Dirksen E. Bussiere,Peter Finan,Mark Labow,Xiaohong Mao,Lawrence G. Hamann,Brendan D. Manning,Reginald Valdez,Thomas B. Nicholson,Markus Schirle,Mark Knapp,Erin P. Keaney,Leon Murphy +46 more
TL;DR: a4−/− mice exhibit a profound accumulation of iron in splenic macrophages, which are critical for the reutilization of iron from engulfed red blood cells, and a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo is revealed.
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LXH254, a Potent and Selective ARAF-Sparing Inhibitor of BRAF and CRAF for the Treatment of MAPK-Driven Tumors.
Kelli-Ann Monaco,Scott Delach,Jing Yuan,Yuji Mishina,Paul Fordjour,Emma Labrot,Daniel J. McKay,Ribo Guo,Stacy Higgins,Hui Qin Wang,Jinsheng Liang,Karen Bui,John Green,Peter Aspesi,Jessi Ambrose,Felipa A. Mapa,Lesley A. Mathews Griner,Mariela Jaskelioff,John Fuller,Kenneth Crawford,Gwynn Pardee,Stephania Widger,Peter S. Hammerman,Jeffrey A. Engelman,Darrin Stuart,Vesselina G. Cooke,Giordano Caponigro +26 more
TL;DR: L LXH254 as mentioned in this paper is a novel RAF-inhibitor able to inhibit dimerized BRAF and CRAF as well as monomeric BRAF while largely sparing ARAF.
Inhibition of prenylated KRAS in a lipid environment.
Johanna M. Jansen,Charles Wartchow,Wolfgang Jahnke,Susan Fong,Tiffany Tsang,Keith B. Pfister,Tatiana Zavorotinskaya,Dirksen E. Bussiere,Jan Marie Cheng,Kenneth Crawford,Yumin Dai,Dove Jeffrey H,Eric Fang,Yun Feng,Jean-Michel Florent,John Fuller,Alvar D. Gossert,Mohammad Hekmat-Nejad,Chrystèle Henry,Julia Klopp,Lenahan William P,Andreas Lingel,Sylvia Ma,Arndt Meyer,Yuji Mishina,Jamie Narberes,Gwynn Pardee,Savithri Ramurthy,S. Rieffel,Darrin Stuart,Sharadha Subramanian,Laura Tandeske,Stephania Widger,Armin Widmer,Aurélie Winterhalter,Isabel Zaror,Stephen F. Hardy +36 more
TL;DR: The assays described here provide a first-time alignment across biochemical, biophysical, and cellular KRAS assays through incorporation of key physiological factors regulating RAS biology, namely a negatively charged lipid environment and prenylation, into the in vitro assays.
Rationally Designed PI3Kα Mutants to Mimic ATR and Their Use to Understand Binding Specificity of ATR Inhibitors
Yipin Lu,Mark Knapp,Kenneth Crawford,Warne Robert L,Robert Elling,Kelly Yan,Michael Doyle,Gwynn Pardee,Li Zhang,Sylvia Ma,Mulugeta Mamo,Elizabeth Ornelas,Yue Pan,Dirksen E. Bussiere,Johanna M. Jansen,Isabel Zaror,Albert Lai,Paul A. Barsanti,Janet Sim +18 more
TL;DR: The ability to obtain structural and binding data for these PI3Kα mutants, together with their ATR-like inhibitor binding profiles, makes these chimeric PI2Kα proteins valuable model systems for structure-based inhibitor design.
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Baculovirus-driven protein expression in insect cells: A benchmarking study
Peggy Stolt-Bergner,Christian Benda,Tim Bergbrede,Hüseyin Besir,Patrick H.N. Celie,Cindy Chang,David N. Drechsel,Ariane Fischer,Arie Geerlof,Barbara Giabbai,Joop van den Heuvel,Georg Huber,Wolfgang Knecht,Anita Lehner,Regis P. Lemaitre,Kristina Nordén,Gwynn Pardee,Ines Racke,Kim Remans,Astrid Sander,Judith Scholz,Magda Stadnik,Paola Storici,Daniel Weinbruch,Isabel Zaror,Linda H.L. Lua,Sabine Suppmann +26 more
TL;DR: This benchmarking initiative compared the expression and purification results on four selected intracellular proteins and highlighted the significant differences in expression yields obtained using different commercially-packaged baculovirus vectors.
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