The invention provides a lactobacillus acidophilus anti-freeze culture medium and an application method thereof. The culture medium includes the following components by weight: 16-20 g of glucose, 0.4-0.8 g of fructus tribuli polysaccharide, 3-5 g of yeast extract, 4-6 g of sodium chloride, 0.3-0.5 g of mannitol, 0.8-1.2 g of sodium glutamate, 8-12 g of soybean peptone, 0.5-2.0 g of diammonium hydrogen citrate, 0.2-0.5 g of betaine, 1.0-2.5 g of dipotassium phosphate, 0.1-0.2 g of MgSO4, 0.5-1.0 ml of Tween-80 and 1,000 ml of distilled water. The culture medium has the advantages that: after lactobacillus acidophilus is cultured by using the anti-freeze culture medium, the freeze-dry survival rate of the lactobacillus acidophilus can reach up to 79.54 percent, and viable count of fungal powder reaches up to 4.09*10 cfu/g; and the lactobacillus acidophilus MRS (de Man, Rogosa and Sharp) broth is used for culture, so that the freeze-dry survival rate of the prepared fungal powder isonly 49.72 percent, and the viable count is only 1.24*10 cfu/g.

Lactobacillus acidophilus anti-freeze culture medium and application method thereof

The invention aims to provide a method for improving the freeze-drying survival rate of Lactobacillus acidophilus. The method comprises the following steps of: culturing the Lactobacillus acidophilus for 18h at the temperature of 37 DEG C, performing sublethal treatment on the Lactobacillus acidophilus, centrifuging for 10 to 15min at the temperature of 4 DEG C at the rotating speed of 7,000rpm, adding skimmed milk and a phosphate buffer solution into bacterial sludge of the Lactobacillus acidophilus, pre-freezing for 8 to 12h at the temperature of -40 DEG C, and putting into a freeze dryer and freeze-drying for 18 to 24h at the temperature of between -50 and -60 DEG C under the vacuum degree of between 4 and 6Pa to obtain bacterial powder of the Lactobacillus acidophilus. In the method for improving the freeze-drying survival rate of the Lactobacillus acidophilus, a Lactobacillus acidophilus culture solution is subjected to cold shock, heat shock or acid shock treatment first so as to ensure that the Lactobacillus acidophilus induces to synthesize cold shock protein and heat shock protein under adverse circumstances or changes the composition of fatty acid of cell membranes, so that the resistance of the Lactobacillus acidophilus in the subsequent freeze-drying process is improved, and the freeze-drying survival rate and the viable count of the bacterial powder are improved.

Method for improving freeze-drying survival rate of Lactobacillus acidophilus

The invention discloses a protective agent for quantitatively preserving microbial strains, which comprises 0.1 to 10 mass parts of water-soluble sugar, 0.1 to 5 mass parts of defatted milk powder, 0.1 to 20 mass parts of gelatin and 0 to 10 mass parts of active carbon. The protective agent well protects various microbial strains; after the protective agent is mixed with the strains and subjected to freeze-drying, the activity of the strains can be ensured when the strains are preserved at 4-8 DEG C in a dark place; moreover, the biochemical characteristics of microbes cannot be influenced, low-temperature freezing is not required and the operation is convenient. Microbial strain freeze-drying products protected by the protective agent can stably survive at the temperature of 20 DEG C for more than 30 days, the requirement on an external environment is not high, and operation of inoculation and the like is greatly facilitated.

Protective agent for quantitatively preserving microbial strains

The invention discloses a streptococcus thermophilus composite antifreezing factor The streptococcus thermophilus composite antifreezing factor comprises: by mass, 01 to 04 parts of glycine betaine, 3 to 5 parts of sodium chloride, 05 to 3 parts of mannose and 01 to 04 parts of calcium carbonate From the perspective of a medium, a medium is added with the streptococcus thermophilus composite antifreezing factor so that formation and accumulation of freeze-drying protection substances are promoted or the freeze-drying protection substances are transformed into other substances; and a eutectic phenomenon in freeze-drying is inhibited Through the accumulation of the freeze-drying protection substances, the composition of streptococcus thermophilus membrane fatty acid is changed and even an antifreezing protein is produced by induction so that an antifreezing capacity and a survival rate of streptococcus thermophilus are improved in freeze-drying and storage

Streptococcus thermophilus composite antifreezing factor

The invention discloses a measurable rotary evaporator which can be widely applied to measurement for a liquid level during evaporation of a liquid sample in a laboratory. The measurable rotary evaporator comprises an evaporating bottle, an air guide pipe, a flow detection unit and a control unit, wherein the evaporating bottle is connected with the air guide pipe; the air guide pipe is connected to the flow detection unit; and the signal output end of the flow detection unit is connected with the signal input end of the control unit. Based on a circuit design of the flow detection unit and the control unit, the measurable rotary evaporating device is realized.

Measurable rotary evaporator

The invention provides a protective agent in the freeze drying process of Lactobacillus acidophilus, which consists of 70 to 120g/L of defatted milk powder, 40 to 80mL/L of glycerol, 30 to 70g/L of trehalose, 05 to 2g/L of ascorbic acid, 3 to 85g/L of sodium chloride and 0 to 5g/L of gelatin The protective agent can make the survival rate of the Lactobacillus acidophilus subjected to freeze drying reach 983 percent, which is improved by 468 times compared with that (21 percent) of the Lactobacillus acidophilus subjected to the freeze drying without the protective agent; and the bacterium powder activity can reach 1*10 cfu/g

Protective agent in the freeze drying process of Lactobacillus acidophilus

The invention provides a lactobacillus acidophilus composite antifreeze factor and a freeze-drying method. According to a formula, 1 liter of the composite antifreeze factor comprises 2 to 6mL of glycerine, 2 to 8g of sodium chloride, 0 to 4g of trehalose and 0 to 0.8g of alanine. The method comprises the following steps of: adding the composite antifreeze factor into a culture medium for culturing the lactobacillus acidophilus; centrifuging and then collecting the lactobacillus acidophilus, cleaning the lactobacillus acidophilus by using normal saline, centrifuging and then removing clear liquid; and preparing the lactobacillus acidophilus into lactobacillus acidophilus suspension by using the normal saline, then uniformly mixing the lactobacillus acidophilus suspension with a protective agent and finally performing freeze drying. The freeze-drying antifreeze factor of the invention is directly added into the culture medium to prepare lactobacillus acidophilus powder; after freeze drying, the maximum survival rate of the lactobacillus acidophilus reaches 82.75 percent and a powder activity reaches over 1*10 cfu/g; and the survival rate of the lactobacillus acidophilus is over three times the survival rate (40.67 percent) of the lactobacillus acidophilus powder prepared without the antifreeze factor for the freeze drying.

Lactobacillus acidophilus composite antifreeze factor and freeze-drying method

The invention provides a multifunctional rotary evaporating device, comprising a bracket, first condensing equipment and second condensing equipment, an extracting device and a pipeline, a connecting channel, an evaporator and heating equipment, wherein the first condensing equipment and the second condensing equipment are fixedly connected with the bracket; the extracting device and the pipeline are respectively communicated with the first condensing equipment and the second condensing equipment; the connecting channel is communicated with the extracting device and the pipeline simultaneously; the evaporator is communicated with the connecting channel; the heating equipment heats the evaporator; the connecting channel is provided with a first rotating piston at the connecting position with the extracting device; and the connecting channel is provided with a second rotating piston at the connecting position with the pipeline. Therefore, the multifunctional rotary evaporating device not only can carry out extraction, but also can carry out backflow and separation, saves cost and has simple operation.

Multifunctional rotary evaporating device

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Protective agent in the freeze drying process of Lactobacillus acidophilus

Lactobacillus acidophilus composite antifreeze factor and freeze-drying method

Multifunctional rotary evaporating device