A sensor array based on the pattern recognition strategy is good at distinguishing subtle changes of extremely similar mixtures over a wide range of analyte types. Particularly, colorimetric sensor arrays based on gold nanoparticles (AuNPs) have been applied widely in many fields for its simplicity, rapidity, cost-effective production, multi-information output, easy visualization of the color change, and even assay by naked eyes. This comprehensive review focuses on the development and state of the art of AuNPs-based colorimetric sensor arrays. Firstly, this review briefly describes the principle of a sensor array and discusses the design of the cross-reactive sensing elements. Secondly, the popular chemometric and statistical techniques (including hierarchical cluster analysis (HCA), principal component analysis (PCA), and linear discriminant analysis (LDA)) for analyzing the data from a sensor array have also been presented. Thirdly, a variety of applications of AuNPs-based colorimetric sensor arrays published throughout the literature have been reviewed in detail according to different sensing mechanisms (surface plasmon resonance, catalytic activity of mimetic enzyme, and multidimensional information) between AuNPs and target analytes. Finally, the remaining challenges and future trends in the development of colorimetric sensor array based on AuNPs have also been discussed.

Colorimetric sensor array based on gold nanoparticles: design principles and recent advances

We report a strategy enabling ultrasensitive colorimetric detection of 17β-estradiol (E2) in water and urine samples using DNA aptamer-coated gold nanoparticles (AuNPs). Starting from an established sensor format where aggregation is triggered when target-bound aptamers dissociate from AuNP surfaces, we demonstrated that step-change improvements are easily accessible through deletion of excess flanking nucleotides from aptamer sequences. After evaluating the lowest energy two-dimensional configuration of the previously isolated E2 binding 75-mer aptamer (KD ∼25 nM), new 35-mer and 22-mer aptamers were generated with KD’s of 14 and 11 nM by simply removing flanking nucleotides on either side of the inner core. The shorter aptamers were found to improve discrimination against other steroidal molecules and to improve colorimetric sensitivity for E2 detection by 25-fold compared with the 75-mer to 200 pM. In comparing the response of all sequences, we find that the excess flanking nucleotides suppress signal ...

Ultrasensitive colorimetric detection of 17β-estradiol: the effect of shortening DNA aptamer sequences.

https://chemistry-europe.onlinelibrary.wiley.com/doi/pdf/10.1002/anse.202000023

Label-Free Colorimetric Biosensors Based on Aptamers and Gold Nanoparticles: A Critical Review

Herein, a versatile and sensitive colorimetric sensor for Hg2+ based on aptamer-target specific binding and target-mediated growth of AuNPs is reported. The 15 T bases are first designed to detect Hg2+ through T-Hg2+ -T coordination. Aptamer-target binding results in the desorption of the aptamer from AuNP surface, the remaining aptamers adsorbed on AuNP surface trigger the growth of AuNPs with morphologically varied nanostructures, and then different colored solutions are formed. On this occasion, the limit of detection (LOD) of 9.6 × 10-9 m is obtained. The other two aptamer strands (25- and 59-mer) are designed by increasing A bases on either side and both sides of 15 T, respectively. The interaction of the binding domain and Hg2+ makes desorption of 15 T from AuNP surface, whereas excess bases not committed to the binding domain still adsorbed on AuNP surface. These excess bases control the growth of AuNPs, and enhance the sensitivity. The LODs are 4.05 and 3 × 10-9 m for 25- and 59-mer aptamers, respectively. In addition, the 59-mer aptamer system is applied to identify Hg2+ in real river samples, the LOD of 6.2 × 10-9 m is obtained.

Colorimetric Detection of Hg2+ Based on the Growth of Aptamer-Coated AuNPs: The Effect of Prolonging Aptamer Strands

Nanotechnology has illustrated significant potentials in biomolecular-sensing applications; particularly its introduction to anti-doping detection is of great importance. Illicit recreational drugs, substances that can be potentially abused, and drugs with dosage limitations according to the prohibited lists announced by the World Antidoping Agency (WADA) are becoming of increasing interest to forensic chemists. In this review, the theoretical principles of optical biosensors based on noble metal nanoparticles, and the transduction mechanism of commonly-applied plasmonic biosensors are covered. We review different classes of recently-developed plasmonic biosensors for analytic determination and quantification of illicit drugs in anti-doping applications. The important classes of illicit drugs include anabolic steroids, opioids, stimulants, and peptide hormones. The main emphasis is on the advantages that noble metal nano-particles bring to optical biosensors for signal enhancement and the development of highly sensitive (label-free) biosensors. In the near future, such optical biosensors may be an invaluable substitute for conventional anti-doping detection methods such as chromatography-based approaches, and may even be commercialized for routine anti-doping tests.

Noble metal nanostructures in optical biosensors: Basics, and their introduction to anti-doping detection.

The utilization of the plasmonic response of aptamer–gold nanoparticle conjugates (Apt–AuNPs) to design cross-reactive arrays for fingerprint identification of small molecular targets was demonstrated for the first time. Four aptamers with different structural features previously selected to bind different targets were used in combination with AuNPs by adsorbing the DNA on the AuNPs surface. The optimized response of the Apt–AuNPs to the analytes showed that, depending on the specific aptamer used, target binding by the aptamer could result in an increase or decrease of Apt–AuNPs stability. These Apt–AuNPs showed the ability to recognize different analytes with different affinities, generating fingerprints that allowed unambiguous analyte identification with response times in less than fifteen minutes. Importantly, it was observed that it was not necessary to select an aptamer per analyte of interest to generate differentiable signatures, but a subset of aptamers could be used to identify a larger number of analytes. The data was analyzed using principal component analysis, showing efficient clustering of the different datasets for qualitative and quantitative identification. This work opens the door to using these Apt–AuNPs in point of care diagnostics applications where fast sensors with easy to read outputs are needed.

/pdf/plasmonic-aptamer-gold-nanoparticle-sensors-for-small-pwq9500tly.pdf

Plasmonic aptamer-gold nanoparticle sensors for small molecule fingerprint identification.

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Plasmonic aptamer-gold nanoparticle sensors for small molecule fingerprint identification.