Giuliano Sciara
University of Provence
9 Papers
185 Citations
Giuliano Sciara is an academic researcher from University of Provence. The author has contributed to research in topics: Siphoviridae & Bacteriophage P2. The author has an hindex of 7, co-authored 9 publications. Previous affiliations of Giuliano Sciara include Centre national de la recherche scientifique.
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Papers
Structure of lactococcal phage p2 baseplate and its mechanism of activation
Giuliano Sciara,Cecilia Bebeacua,Patrick Bron,Denise M. Tremblay,Miguel Ortiz-Lombardía,Julie Lichière,Marin van Heel,Valérie Campanacci,Sylvain Moineau,Christian Cambillau +9 more
TL;DR: Analysis of the p2 phage baseplate structure by X-ray crystallography and electron microscopy and propose a mechanism for the baseplate activation during attachment to the host cell reveal a novel siphophage activation and host-recognition mechanism leading ultimately to DNA ejection.
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There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization.
TL;DR: The structure of an Escherichia coli contaminating protein, acriflavine resistance protein B (AcrB), is compared with previously deposited AcrB structures and strategies are proposed to avoid this contamination.
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Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1.
Kerstin Michalke,Céline Huyghe,Julie Lichière,Marie-Eve Gravière,Marina Isabella Siponen,Giuliano Sciara,Isabelle Lepaul,Renaud Wagner,Christine Magg,Rainer Rudolph,Christian Cambillau,Aline Desmyter +11 more
TL;DR: A novel artificial chaperone-assisted refolding procedure adapted for the GPCR inclusion body refolding, followed by protein purification and characterization that demonstrates excellent affinity of both refolded and purified receptors for their respective ligands.
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Solution and electron microscopy characterization of lactococcal phage baseplates expressed in Escherichia coli.
Valérie Campanacci,David Veesler,Julie Lichière,Stéphanie Blangy,Giuliano Sciara,Sylvain Moineau,Douwe van Sinderen,Douwe van Sinderen,Patrick Bron,Christian Cambillau +9 more
TL;DR: A "block cloning" expression strategy was revisited and extended to genomic fragments encoding proteins whose interacting partners have not yet been clearly identified to highlight similarities and differences with the well characterized much larger baseplate of the myophage T4.
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Crystal Structure of ORF12 from Lactococcus lactis Phage p2 Identifies a Tape Measure Protein Chaperone
Marina Siponen,Giuliano Sciara,Manuela Villion,Silvia Spinelli,Julie Lichière,Christian Cambillau,Sylvain Moineau,Valérie Campanacci +7 more
TL;DR: It is suggested that ORF12 might act as a chaperone for TMP hydrophobic repeats, maintaining TMP in solution during the tail assembly of the lactococcal siphophage p2.