1. The effects of the general anaesthetic 2,6-diisopropylphenol (DIP) on synaptic transmission and the actions of amino acid transmitter candidates have been investigated in rat olfactory cortex slices. 2. On electrical stimulation of the lateral olfactory tract (LOT), DIP (20 to 200 microM) increased the area of those surface field potentials which reflect gamma-aminobutyric acid (GABA)-mediated transmission in a concentration-dependent manner in 6 out of 12 slices. In a series of conditioning experiments, DIP (50 microM) also potentiated GABA-mediated pre- and post-synaptic inhibition. 3. Perfusion of slices with DIP (50 microM) potentiated the reduction in the excitability of the terminals of the LOT produced by exogenous GABA in a picrotoxin-sensitive manner. 4. DIP (50 microM) markedly potentiated the surface depolarizations evoked by GABA, muscimol and 3-aminopropanesulphonic acid. The effect on the response to 3-aminopropanesulphonic acid was observed over a concentration range of DIP of 6.25 to 50 microM and was not blocked by the benzodiazepine receptor antagonist Ro 15-1788. 5. In slices in which GABA-mediated transmission was abolished by picrotoxin (25 microM), DIP (50 microM) had no significant effect on monosynaptically-evoked excitatory transmission but depressed the areas of those field potentials which reflect di-/polysynaptic excitations in a concentration-dependent manner (from between 1.6 and 6.25 to 50 microM). 6. In a series of conditioning experiments DIP (50 microM) abolished the increase in the excitability of the pyramidal cells evoked on stimulation of deep association fibres. 7. DIP (50 microM) had no significant effect on surface depolarizations evoked by N-methyl-D-aspartate, quisqualate and kainate or by the transmitter candidates L-glutamate and L-aspartate. 8. It is concluded that, at clinically relevant concentrations, DIP potentiates GABA-mediated transmission probably by an interaction with the GABA receptor complex and inhibits di-/polysynaptic excitations, possibly by inhibiting the release of excitatory transmitters.

Effects of the anaesthetic 2,6-diisopropylphenol on synaptic transmission in the rat olfactory cortex slice.

1 The actions of the endogenous excitatory amino acids (EAAS) glutamate (Glu), aspartate (Asp) and homocysteate (HCA) on Purkinje cells and neurones of the deep nuclei in cerebellar slice cultures were investigated using intracellular recordings in the single-electrode voltage-clamp mode and the whole-cell configuration of the patch-clamp technique 2 Purkinje cells and neurones of deep cerebellar nuclei were identified according to their localization in the living cultures, their morphology as revealed by intracellular injections of Lucifer Yellow and their immunoreactivity to antibodies to the 28 kDa Ca2(+)-binding protein 3 When Purkinje cells were voltage-clamped near their resting membrane potential in a TTX-containing salt solution, Glu, Asp and HCA induced inward currents which were abolished by 6-cyano-7-nitroxaline-2,3-dione (CNQX), a selective antagonist of the non-N-methyl-D-aspartate (NMDA) subtype of EAA receptors The selective antagonist of NMDA receptors, D-(-)-2-amino-5-phosphonovaleric acid (D-APV), was ineffective in blocking the responses induced by these three amino acids NMDA, even at high concentrations and in magnesium-free bathing solution, had no detectable effect on membrane properties of Purkinje cells grown in culture during 11-34 days 4 In magnesium-containing saline, the amplitude of the responses induced by Glu, Asp and HCA was a linear function of the membrane potential 5 In contrast, neurones of the deep cerebellar nuclei were responsive to NMDA and the inward currents induced by Glu, Asp and HCA were partially blocked both by CNQX and by D-APV 6 In magnesium-containing saline, the amplitude of the currents induced by NMDA as well as by the three endogenous EAAs decreased at hyperpolarizing holding potentials whereas the current-voltage relation of the responses induced by quisqualate (QA) was strictly linear 7 It is concluded that Purkinje cells in cerebellar slice cultures do not express NMDA receptors and that excitation of these neurones by the endogenous amino acids Glu, Asp and HCA is mediated exclusively through the activation of non-NMDA receptors In the same preparation, neurones of the deep cerebellar nuclei possess NMDA and non-NMDA receptors which can be both activated by the three endogenous excitatory amino acids

Responses to excitatory amino acids of Purkinje cells' and neurones of the deep nuclei in cerebellar slice cultures.

1 The effect of 2-amino-4-phosphonobutyrate (APB) on facilitation at the lateral olfactory tract (LOT)-superficial pyramidal cell synapse of the olfactory cortex has been studied by recording the relative changes in amplitude of the N-waves evoked on stimulation of the LOT by pairs of stimuli. 2 Although APB (0.01 to 5 mM) reduced the amplitude of the conditioning response there was an overall increase in facilitation over conditioning intervals of up to 1700 ms which was concentration-dependent and inversely related to the concentration of extracellular calcium (1.25 to 5 mM). 3 The L-(+)-isomer of APB was more potent than the D-(-)-form in increasing synaptic facilitation. 4 The potassium channel blockers 4-aminopyridine (0.25 mM), 3,4-diaminopyridine (0.1 mM), tetraethylammonium (10 mM) and catechol (1 mM) all reduced facilitation but failed to antagonize the increase in facilitation produced by APB (1 mM). In contrast, all 4 drugs antagonized APB-induced reductions in the amplitude of the conditioning response. 5 APB (1 mM) significantly reduced the K+-evoked release of endogenous aspartate and glutamate but not of gamma-aminobutyric acid from slices of olfactory cortex. 6 It is suggested that APB reduces the amplitude of the conditioning response and increases synaptic facilitation by reducing transmitter release from the LOT terminals. The mechanism is unlikely to involve activation of terminal potassium currents.

Possible presynaptic actions of 2-amino-4-phosphonobutyrate in rat olfactory cortex.

Thoracic radiotherapy for limited-stage small cell lung cancer: Issues of timing, volumes, dose, and fractionation ☆

L-homocysteic acid mediates synaptic excitation at NMDA receptors in the hippocampus.

The impact of virtual simulation in palliative radiotherapy for non-small-cell lung cancer.

A quantitative estimate of the contribution made by various receptor categories to the depolarizations evoked by some excitatory amino acids in the olfactory cortex

P1-066: Image acquisition protocol to optimize image registration of lung cancer hyperpolarized helium-3 MRI and CT

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Gillian Brown

Author Tools

Chat about Author

Papers

The impact of virtual simulation in palliative radiotherapy for non-small-cell lung cancer.

A quantitative estimate of the contribution made by various receptor categories to the depolarizations evoked by some excitatory amino acids in the olfactory cortex

P1-066: Image acquisition protocol to optimize image registration of lung cancer hyperpolarized helium-3 MRI and CT