Gianluca Zambanini
12 Papers
1 Citations
Gianluca Zambanini is an academic researcher. The author has contributed to research in topics: Biology & Wnt signaling pathway. The author has an hindex of 2, co-authored 6 publications.
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Papers
The CUT&RUN suspect list of problematic regions of the genome
TL;DR: It is proposed that removing problematic regions of high and unstructured signal can substantially improve peak calling in CUT&RUN experiments, resulting in more reliable datasets.
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The time-resolved genomic impact of Wnt/β-catenin signaling.
Pierfrancesco Pagella,Simon Söderholm,Anna Nordin,Gianluca Zambanini,Valeria Ghezzi,Amaia Jauregi-Miguel,Claudio Cantù +6 more
TL;DR: Using a combination of time-resolved CUT&RUN against β-catenin, ATAC-seq, and perturbation assays in different cell types, Wang et al. as mentioned in this paper showed that Wnt/β-Catenin physical targets are tissue-specific, β-CatENin "moves" on different loci over time, and its association to DNA accompanies changing chromatin accessibility landscapes that determine cell behavior.
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The CUT&RUN Blacklist of Problematic Regions of the Genome
TL;DR: In this paper , the ENCODE project and others have compiled blacklists for ChIP-seq which have been widely adopted: these lists contain regions of high and unstructured signal, regardless of cell type or protein target.
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A New CUT&RUN Low Volume-Urea (LoV-U) protocol uncovers Wnt/β-catenin tissue-specific genomic targets
TL;DR: A biochemical modification of the CUT&RUN protocol is presented, which is referred to as LoV-U (Low Volume and Urea), that enables the generation of robust and reproducible β-catenin binding profiles and uncovers direct WNT/β- catenin target genes in human cells, as well as in ex vivo cells isolated from developing mouse tissue.
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The Developmental Transcription Factor TBX3 Physically Engages with the Wnt/β-catenin Transcriptional Complex in Human Colorectal Cancer Cells to Regulate Metastasis Genes
Amaia Jauregi-Miguel,Simon Söderholm,Tamina Weiss,Anna Nordin,Valeria Ghezzi,Salome Brütsch,Pierfrancesco Pagella,Yorick Bernardus Cornelis van de Grift,Gianluca Zambanini,Jacopo Ulisse,Alessandro Mattia,Ruslan Deviatiiarov,Elena Faustini,Lavanya Moparthi,Francisca Lottersberger,Stefan Koch,Andreas E. Moor,Xiao-Feng Sun,Eleonore von Castelmur,Guojun Sheng,Claudio Cantù +20 more
TL;DR: TBX3 emerges as a key modulator of the oncogenic activity of Wnt/β-catenin in colorectal cancer, and its mechanism of action exposes a novel druggable protein-interaction surface.
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