Gavin E. Murphy

TL;DR: These protocols for electron cryotomography sample preparation are described including introduction of fiducial markers into the sample and sample vitrification, which almost exclusively use an automated, climate-controlled plunge-freezing device (the FEI Vitrobot) to vitrify samples.

TL;DR: Qualitative advances that extend the current capabilities of ion-abrasion scanning electron microscopy (IA-SEM) are reported and it is demonstrated that by combining correlative imaging with newly developed tools for automated image processing, small 100nm-sized entities can be localized in SEM image stacks of whole mammalian cells.

TL;DR: 3D architectures of contact regions between HIV-1-infected CD4+ T cells and either uninfected human CD4-T cells or human fetal astrocytes are determined, showing that individual virions are distributed along the length ofAstrocyte filopodia, suggesting that virus transfer to the astroCytes is mediated, at least in part, by processes originating from the astROcyte itself.

TL;DR: The design, characterization, and demonstration of a new "flip-flop" rotation stage that allows facile and routine collection of two orthogonal tilt-series of cryosamples and suggests that further improvement could be realized, however, through better software to align and merge dual-axis tilt- series of cryOSamples.