Gabriele Pohlig
Novartis
8 Papers
101 Citations
Gabriele Pohlig is an academic researcher from Novartis. The author has contributed to research in topics: Saccharomyces cerevisiae & Protease. The author has an hindex of 7, co-authored 8 publications.
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Papers
A new structural class of serine protease inhibitors revealed by the structure of the hirustasin–kallikrein complex
Peer R. E. Mittl,Stefania Di Marco,Gabriele Fendrich,Gabriele Pohlig,Jutta Heim,Christian P. Sommerhoff,Hans Fritz,John P. Priestle,Markus G. Grütter +8 more
TL;DR: It is revealed that hirustasin differs from other serine protease inhibitors in its conformation and its disulfide bond connectivity, making it the prototype for a new class of inhibitor.
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C‐Terminal Proteolytic Degradation of Recombinant Desulfato‐Hirudin and Its Mutants in the Yeast Saccharomyces cerevisiae
TL;DR: It was concluded that ysc alpha might also be responsible for the C-terminal degradation of recombinant atrial natriuretic factor and epidermal growth factor expressed in yeast.
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Purification, characterization and biological evaluation of recombinant leech-derived tryptase inhibitor (rLDTI) expressed at high level in the yeast Saccharomyces cerevisiae.
Gabriele Pohlig,Gabriele Fendrich,René Knecht,Beate Eder,Gerd P. Piechottka,Christian P. Sommerhoff,Jutta Heim +6 more
TL;DR: Recombinant LDTI appears to be as potent as the authentic leech protein, exhibiting Ki-values of approximately 1.5 nM and Approximately 1.6 nM against human tryptase and bovine trypsin and to interfere with thetryptase-induced proliferation of human fibroblasts and keratinocytes.
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2-micron vectors containing the Saccharomyces cerevisiae metallothionein gene as a selectable marker: excellent stability in complex media, and high-level expression of a recombinant protein from a CUP1-promoter-controlled expression cassette in cis.
Thomas Dr. Hottiger,Jochen Kuhla,Gabriele Pohlig,Peter Fürst,Albert Spielmann,M.B. Garn,Stephan Haemmerli,Jutta Heim +7 more
TL;DR: The results clearly establish the utility of the CUP1 marker in the construction of stable yeast expression vectors and allow for the production of very high amounts of biologically active hirudin in a copper‐sensitive host strain.
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Recombinant hirustasin: production in yeast, crystallization, and interaction with serine proteases.
S. Di Marco,Gabriele Fendrich,René Knecht,André Strauss,Gabriele Pohlig,Jutta Heim,John P. Priestle,Christian P. Sommerhoff,Markus G. Grütter +8 more
TL;DR: Hirustasin appears to fall into the class of protease inhibitors displaying temporary inhibition, and could be processed in vitro using a soluble variant of the yeast yscF protease.
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