In the cell, as in vitro, the final conformation of a protein is determined by its amino-acid sequence. But whereas some isolated proteins can be denatured and refolded in vitro in the absence of other macromolecular cellular components, folding and assembly of polypeptides in vivo involves other proteins, many of which belong to families that have been highly conserved during evolution.

Protein folding in the cell.

Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes

https://www.cell.com/trends/immunology/pdf/0167-5699(92)90111-J.pdf

The mechanism of action of cyclosporin A and FK506

A nuclear magnetic resonance (NMR)-based method is described in which small organic molecules that bind to proximal subsites of a protein are identified, optimized, and linked together to produce high-affinity ligands. The approach is called "SAR by NMR" because structure-activity relationships (SAR) are obtained from NMR. With this technique, compounds with nanomolar affinities for the FK506 binding protein were rapidly discovered by tethering two ligands with micromolar affinities. The method reduces the amount of chemical synthesis and time required for the discovery of high-affinity ligands and appears particularly useful in target-directed drug research.

https://science.sciencemag.org/content/sci/274/5292/1531.full.pdf

Discovering High-Affinity Ligands for Proteins: SAR by NMR

(2002). The Effects of Harmful Algal Blooms on Aquatic Organisms. Reviews in Fisheries Science: Vol. 10, No. 2, pp. 113-390.

The Effects of Harmful Algal Blooms on Aquatic Organisms

bromide with NBS and debromination with zinc-copper couple, potassium iodide, and iodine in D M F (24%).69i6 Again, C,, symmetry was evident from the N M R spectra.\" The colorless crystalline substance (mp 27-29 \"C) exhibits the following electronic spectrum: A, , cyclohexane 218 (c 8900) and 318 (3000). Photoelectron spectroscopic measurements on 1 will be reported elsewhere. Heating a CDC13 solution of 1 to 80 O C induces clean rearrangement to 1618 ( t l -7 h). The same isomerization can be achieved more rapicily by irradiation with a TLC UV lamp (A 254 nm). Accordingly, 1 finds [1,3] sigmatropic migration to be most accessible from its ground and excited states.

Structure of brevetoxin A (GB-1 toxin), the most potent toxin in the Florida red tide organism Gymnodinium breve (Ptychodiscus brevis)

Atomic Structure of the Rapamycin Human Immunophilin FKBP-12 Complex

Substituent effects on the alkylations of the dimethylhydrazones of 2,4- and 2,6-disubstituted cyclohexanones are reported. High axial selectivities are observed in the alkylations of cyano-substituted metalated hydrazones, but not with alkoxycarbonyl-substituted cases. Hydrazones of 2-substituted cyclohexanones appear to alkylate axially out of a conformer with the 2-substituent pseudoaxially disposed. The possible origins of the stereoselectivities are addressed in light of an X-ray crystal structure of lithiated cyclohexanone dimethylhydrazone.

Substituent effects on the stereochemistry of substituted cyclohexanone dimethylhydrazone alkylations. An x-ray crystal structure of lithiated cyclohexanone dimethylhydrazone

Structure of the psammaplysins

An unprecedented triisocyano diterpenoid antibiotic from a sponge

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

G. D. Van Duyne

Author Tools

Chat about Author