An endothelium-derived 21-residue vasoconstrictor peptide, endothelin, has been isolated, and shown to be one of the most potent vasoconstrictors known. Cloning and sequencing of preproendothelin complementary DNA shows that mature endothelin is generated through an unusual proteolytic processing, and regional homologies to a group of neurotoxins suggest that endothelin is an endogenous modulator of voltage-dependent ion channels. Expression of the endothelin gene is regulated by several vasoactive agents, indicating the existence of a novel cardiovascular control system.

/pdf/a-novel-potent-vasoconstrictor-peptide-produced-by-vascular-4zvh0kyupn.pdf

A novel potent vasoconstrictor peptide produced by vascular endothelial cells.

Publisher Summary This chapter describes techniques concerned with classical and molecular genetics, cell biology, and biochemistry that can be used with Schizosaccharomyces pombe . Conjugation and sporulation cannot take place in S. pombe except under conditions of nutrient starvation. ME medium is generally used for genetic crosses. To cross two strains, a loopful of h – and a loopful of h + are mixed together on a ME plate. The cross is left to dry and is then incubated below 30°, as conjugation is severely reduced above this temperature. Fully formed four-spore asci can be seen after 2–3 days of incubation. A 2 day-old cross is usually used for tetrad analysis. Using a 3-day-old cross, one can check for the presence of asci under the light microscope. Random spore analysis allows many more spores to be examined than in tetrad analysis, and in this way recombination mapping and strain construction can be carried out. Diploid cells arise spontaneously in most S. pombe strains, this characteristic can be used to isolate homozygous diploids of any strain. For mutagenesis of yeast strains, ethylmethane sulfonate (EMS) and nitrosoguanidine is used.

Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.

Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation.

Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid

https://www.cell.com/cell/pdf/0092-8674(92)90452-I.pdf

Influenza virus M2 protein has ion channel activity.

https://onlinelibrary.wiley.com/doi/pdf/10.1016/0307-4412%2885%2990051-2

Transcription and Translation — A Practical Approach: Edited by B D Hames and S J Higgins. pp 328. IRL Press, Oxford. 1984. £12. ISBN 0-904147-52-5

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Transcription and Translation — A Practical Approach: Edited by B D Hames and S J Higgins. pp 328. IRL Press, Oxford. 1984. £12. ISBN 0-904147-52-5