Fumitake Usui
Shinshu University
11 Papers
40 Citations
Fumitake Usui is an academic researcher from Shinshu University. The author has contributed to research in topics: Population & Bone marrow. The author has an hindex of 7, co-authored 11 publications.
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Papers
Migration and Proliferation of Primordial Germ Cells in the Early Chicken Embryo
Yoshiaki Nakamura,Yasuhiro Yamamoto,Fumitake Usui,T. Mushika,Tamao Ono,A. R. Setioko,K. Takeda,Keijiro Nirasawa,Hiroshi Kagami,Takahiro Tagami +9 more
TL;DR: It is clarified that chicken PGC continue to proliferate throughout early development, many PGC invaded into the vascular system from the region anterior to the head in stage 11, and PGC actively left the blood vessels and migrated to the intermediate mesoderm from stage 15.
159
A Novel Method to Isolate Primordial Germ Cells and Its Use for the Generation of Germline Chimeras in Chicken
Yasuhiro Yamamoto,Fumitake Usui,Yoshiaki Nakamura,Yohei Ito,Takahiro Tagami,Keijiro Nirasawa,Yuko Matsubara,Tamao Ono,Hiroshi Kagami +8 more
TL;DR: The results of the present study suggest that this novel method for the isolation of circulating PGCs will become a powerful tool for germline manipulation in the chicken.
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Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos.
Yoshiaki Nakamura,Fumitake Usui,Yusuke Atsumi,Asako Otomo,Ayumi Teshima,Tamao Ono,Kumiko Takeda,Keijiro Nirasawa,Hiroshi Kagami,Takahiro Tagami +9 more
TL;DR: In conclusion, injecting busulfan sustained-release emulsion into the yolk of recipient embryos at 0h of incubation was a suitable method of preparing them for the generation of germline chimeras.
22
Depletion of primordial germ cells (PGCs) by x-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs.
Yusuke Atsumi,Shigenobu Yazawa,Fumitake Usui,Yoshiaki Nakamura,Yasuhiro Yamamoto,Yasuhiro Yamamoto,Takahiro Tagami,Kohzy Hiramatsu,Hiroshi Kagami,Tamao Ono +9 more
TL;DR: Exposure of chicken embryos to ∼7.2Gy of x-radiation at stage 13 with the application of a lead shield to the embryo proper is thus a feasible approach to depletion of endogenous germ cells and the production of chicken-quail germline chimeras.
9
Analysis of developmental changes in avian DNA methylation using a novel method for quantifying genome-wide DNA methylation.
TL;DR: In this article, the authors show that the DNA methylation status at stage 1 was approximately 57%, after which it gradually decreases, reaching a minimum at stage 10 (33%), after stage 10, DNA methylisation gradually increased.
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