A method and reagent system is described for differential determination of more that one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolyzing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolyzing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is a non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolyzation.

Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes

A reagent, satisfying the undermentioned conditions, is useful for counting the number of leukocytes and measuring the hemoglobin concentration in blood samples. The reagent is free of cyanides and it is able to maintain hemoglobin in a stable state for a prolonged time. The reagent contains at least one cationic surfactant selected from the group consisting of a quaternary ammonium salt of a concentration capable of hemolyzing erythrocytes in the blood and oxidizing hemoglobin and a composition of pyridinium salts; at least one cationic, nonionic or amphoteric surfactant; and at least one hemoglobin stabilizer.

Reagent for measurement of leukocytes and hemoglobin in blood

A reagent for measuring the hemoglobin concentration and counting the number of leukocytes in a blood sample, which contains at least one of a quaternary ammonium salt or a pyridinium salt, having a concentration capable of hemolyzing erythrocytes in the blood and denaturing hemoglobin and at least one of cationic, nonionic or amphoteric surfactants or an oxidant capable of oxidizing heme in hemoglobin, the reagent being capable of dividing leukocytes into two or three fractions, for example, a fraction of lymphocytes, a fraction of monocytes, eosinophils and basophils, and a fraction of neutrophils.

Reagent for simultaneous determination of hemoglobin and leukocytes in blood

A method and reagent system for differential determination of more than one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolysing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolysing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolysation.

Method and stromatolysing reagent for the volumetric determination of populations of leukocytes

An immunologic assay for determining the presence of one or more phospholipids in a biological fluid. The method includes adding an ethanolic solution of diacylphosphatidylcholine or alkyltrimethylammonium halide and cholesterol to the biological fluid forming a macromolecular aggregate complex suspension. To the macromolecular aggregate complex solution is then added either an additional quantity of the biological fluid or a buffer reagent. The product of the reaction is then added to antibody molecules to the phospholipid and examined to determine the presence of the phospholipids. The method is particularly useful in determining the presence of phosphatidylglycerol in a sample of amniotic fluid.

Immunoassay of phospholipid, such as phosphatidyl choline, in fluids such as amniotic

A method of reducing scattered light during photometric measurements, especially during the photometric measurement of haemoglobin and a reagent for the performance of the method. To a suspension which is ready for a measurement operation there is admixed an additive which reduces the difference between the refractive index of the suspension and the mean refractive index of the suspended phase. The reagent for the photometric determination of the haemoglobin comprises a lysing agent, means for modifying the refractive index, means for stabilizing the leukocytes and a solvent. The reagent is admixed as an additive to a suspension of blood particles in an isotonic solvent.

Reducing light scatter in photometric measurements involving suspended particles

Die Erfindung betrifft ein Verfahren zur Verminderung des Streulichts bei photometrischen Messungen, insbesondere bei der photometrischen Messung von Hamoglobin und ein Reagens zur Durchfuhrung des Verfahrens. The invention relates to a method for reducing the scattered light for photometric measurements, in particular in the photometric measurement of hemoglobin, and a reagent for carrying out the method. Einer messbereiten Suspension wird ein Zusatz beigemischt, der die Differenz zwischen, dem Brechungsindex des Suspensionsmittels und dem mittleren Brechungsindex der suspendierten Phasen verkleinert. A measuring prepare suspension is admixed with an additive which reduces the difference between, the refractive index of the suspension medium and the average refractive index of the suspended phases. Ein Reagens zur photometrischen Bestimmung von Hamoglobin besteht aus einem Lysiermittel, einem Mittel zum Modifizieren des Brechungsindexes, einem Mittel zum Stabilisieren der Leukozyten und einem Losungsmittel. A reagent for the photometric determination of hemoglobin consists of a lysing agent, an agent for modifying the refractive index, an agent for stabilizing the leucocytes and a solvent. Das Reagens wird als Zusatz einer Suspension von Blutkorperchen in einem isotonischen Losungsmittel beigemischt. The reagent is admixed as an additive to a suspension of blood corpuscles in an isotonic solvent.

Method for the reduction of the disturbance of photometric measurements by light scatter in a suspension to be measured and reagent for carrying out the method

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Reducing light scatter in photometric measurements involving suspended particles

Method for the reduction of the disturbance of photometric measurements by light scatter in a suspension to be measured and reagent for carrying out the method