Frederick A. Fletcher

TL;DR: Several replication-defective retrovirus vectors were tested for their ability to transfer and express human adenosine deaminase in vitro and in vivo in a mouse bone marrow transplantation model, offering the opportunity to assess methods for increasing efficiency of gene transfer, for regulation of expression of foreign genes in hematopoietic progenitors, and for long-term measurement of the stability of expression in these cells.

TL;DR: Analyses of flt3 ligand cDNA clones show that alternative splicing of a putative sixth exon results in the generation of a soluble form of the flt 3 ligand protein.

TL;DR: The vector did not contain a selectable marker, and the infected stem cells did not have a competitive in vivo advantage, Nevertheless, it was observed consistent gene transfer into hematopoietic stem cells and long-term expression of a human gene product in their progeny.

TL;DR: Recombinant proteins expressed from the rat cDNAs bind to elk with high affinity, similar to recombinant human LERK-2 and an endogenously-expressed rat elk-binding protein.