Franziska Weichmann
University of Regensburg
4 Papers
Franziska Weichmann is an academic researcher from University of Regensburg. The author has contributed to research in topics: RNA splicing & RNA. The author has an hindex of 4, co-authored 4 publications.
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Papers
Interactions, localization, and phosphorylation of the m6A generating METTL3-METTL14-WTAP complex.
Eva Schöller,Franziska Weichmann,Thomas Treiber,Sam Ringle,Nora Treiber,Andrew Flatley,Regina Feederle,Astrid Bruckmann,Gunter Meister +8 more
TL;DR: It is confirmed that monomeric METTL3 is soluble and inactive while the catalytic center of METTL14 is degenerated and thus also inactive and it is shown that the C-terminal RGG repeats ofMETTL14 are required forMETTL3/14 activity by contributing to RNA substrate binding.
Comprehensive analysis of translation from overexpressed circular RNAs reveals pervasive translation from linear transcripts.
Hung Ho-Xuan,Petar Glažar,Claudia Latini,Kevin Heizler,Jacob Haase,Robert Hett,Marvin Anders,Franziska Weichmann,Astrid Bruckmann,Debbie L. C. van den Berg,Stefan Hüttelmaier,Nikolaus Rajewsky,Christina Hackl,Gunter Meister +13 more
TL;DR: It is reported that the ORF of circZNF609 is efficiently translated when expressed from a circZ NF609 overexpression construct, however, endogenous proteins could not be detected and underline that circRNA overexposure constructs need to be evaluated carefully, particularly when functional studies are performed.
Validation strategies for antibodies targeting modified ribonucleotides.
Franziska Weichmann,Robert Hett,Aloys Schepers,Taku Ito-Kureha,Andrew Flatley,Kaouthar Slama,Florian D. Hastert,Nicholas B. Angstman,M. Cristina Cardoso,Julian König,Stefan Hüttelmaier,Christoph Dieterich,Stefan Canzar,Mark Helm,Vigo Heissmeyer,Regina Feederle,Gunter Meister +16 more
TL;DR: Here, a experimental toolbox is provided that allow for testing antibody performance prior to their use before they are used and is critical that the affinity and specificity is rigorously tested using complementary approaches.
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Determination of enrichment factors for modified RNA in MeRIP experiments.
Kaouthar Slama,Aurellia Galliot,Franziska Weichmann,Jasmin Hertler,Regina Feederle,Gunter Meister,Mark Helm +6 more
TL;DR: A method to assess enrichment factors after MeRIP-type pulldown experiments, exemplified with a commercial antibody against N6-methyladenosine (m6A) to measure enrichment factors of 4-5 using m6A-containing mRNAs against an unmodified control of identical sequence.