Francis M. Mann
Winona State University
19 Papers
50 Citations
Francis M. Mann is an academic researcher from Winona State University. The author has contributed to research in topics: Chemistry & Mycobacterium tuberculosis. The author has an hindex of 11, co-authored 19 publications. Previous affiliations of Francis M. Mann include University of Wisconsin–Parkside & Iowa State University.
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Papers
Diterpene cyclases and the nature of the isoprene fold.
Rong Cao,Yonghui Zhang,Francis M. Mann,Cancan Huang,Dushyant Mukkamala,Michael P. Hudock,Matthew E. Mead,Sladjana Prisic,Ke Wang,Fu Yang Lin,Ting Kai Chang,Reuben J. Peters,Eric Oldfield +12 more
TL;DR: Structural models based on bioinformatics, site‐directed mutagenesis, domain swapping, enzyme inhibition, and spectroscopy are proposed that help explain the nature of diterpene cyclase structure, function, and evolution.
Insights into Diterpene Cyclization from Structure of Bifunctional Abietadiene Synthase from Abies grandis
TL;DR: Visualization of the class I and II active sites confirms known and implicates new determinants of product formation and assigns specific roles in substrate binding and catalysis to residues, previously unrecognized.
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Edaxadiene: A New Bioactive Diterpene from Mycobacterium tuberculosis
TL;DR: The characterization of an atypical class I diterpene cyclase from M. tuberculosis that catalyzes an unusual cyclization reaction in converting the known M.culosis metabolite halimadienyl diphosphate to a further cyclized novel diterPene, which is termed edaxadiene, as it directly inhibits maturation of the phagosomal compartment in which the bacterium is taken up during infection.
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A Single Residue Switch for Mg2+-dependent Inhibition Characterizes Plant Class II Diterpene Cyclases from Primary and Secondary Metabolism
Francis M. Mann,Sladjana Prisic,Emily K. Davenport,Mara K. Determan,Robert M. Coates,Reuben J. Peters +5 more
TL;DR: A conserved basic residue is discovered that seems to act as a counter ion to the DXDD motif, enhancing the ability of aspartic acid to carry out the requisite energetically difficult protonation of a carbon-carbon double bond and also affecting inhibitory Mg2+ binding.
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Characterization and Inhibition of a Class II Diterpene Cyclase from Mycobacterium tuberculosis IMPLICATIONS FOR TUBERCULOSIS
TL;DR: It is hypothesized that the inability to produce edaxadiene may be a contributing factor in the decreased infectivity and/or virulence of M. bovis relative to M. tuberculosis in humans.
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