Florian David
Chalmers University of Technology
52 Papers
123 Citations
Florian David is an academic researcher from Chalmers University of Technology. The author has contributed to research in topics: Saccharomyces cerevisiae & Biology. The author has an hindex of 19, co-authored 43 publications. Previous affiliations of Florian David include Braunschweig University of Technology.
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Papers
EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae
Niels Bjerg Jensen,Tomas Strucko,Kanchana Rueksomtawin Kildegaard,Florian David,Jerome Maury,Uffe Hasbro Mortensen,Jochen Förster,Jens Nielsen,Jens Nielsen,Irina Borodina +9 more
TL;DR: It is demonstrated that selective markers can be simultaneously removed using Cre-mediated recombination and all the integrated heterologous genes remain in the chromosome and show unchanged expression levels, suitable for metabolic engineering in yeast where multiple rounds of gene introduction and marker recycling can be carried out.
301
Reprogramming Yeast Metabolism from Alcoholic Fermentation to Lipogenesis.
Tao Yu,Yongjin J. Zhou,Mingtao Huang,Quanli Liu,Rui Pedro Gomes Pereira,Florian David,Jens Nielsen,Jens Nielsen +7 more
TL;DR: It is shown through metabolic engineering that it is possible to alter the metabolism of Saccharomyces cerevisiae from traditional ethanol fermentation to a pure lipogenesis metabolism, resulting in high-level production of free fatty acids.
272
Impact of synthetic biology and metabolic engineering on industrial production of fine chemicals
TL;DR: Some of the bio-based fine chemicals that have reached the market, key metabolic engineering tools that have allowed this to happen and some of the companies that are currently utilizing these technologies for developing industrial production processes are described.
241
Flux Control at the Malonyl-CoA Node through Hierarchical Dynamic Pathway Regulation in Saccharomyces cerevisiae
TL;DR: A novel approach for dynamic modulation of pathway flux and enzyme expression levels around the key pathway intermediate malonyl-CoA is presented, which increased the production of 3-HP by 10-fold and can be applied for production of other malony l-coA-derived products.
142
Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation Using Csy4 in Saccharomyces cerevisiae
TL;DR: It is demonstrated that multiplexed genome editing and gene regulation can be performed in a fast and effective manner using Csy4.
99