Florence Chua
National University of Singapore
5 Papers
202 Citations
Florence Chua is an academic researcher from National University of Singapore. The author has contributed to research in topics: Osmotic concentration & Osmole. The author has an hindex of 4, co-authored 5 publications.
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Papers
Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate.
TL;DR: This study demonstrates that hybridomas can be hyperstimulated to produce higher immunoglobulin (lg) levels by suppressing cell growth and increasing culture longevity through adaptation to higher osmolarity media and addition of sodium butyrate.
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Hyper-stimulation of monoclonal antibody production by high osmolarity stress in eRDF medium
TL;DR: Study on the mechanism of action of osmotic stress on hybridoma 2HG11 revealed that hyper-stimulation of Ig productivity was fundamentally related to a greater availability of amino acids to cells as the cells actively accumulated more salt and amino acid to compensate for the higher medium osmolarity.
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Enhanced IgG production in eRDF media with and without serum. A comparative study.
TL;DR: The manufacturing cost (excluding purification) of Ig using eRDF was calculated to be between 17-50% of the price of the other two media and therefore this is regarded as the best medium for Ig production.
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Overproduction Of Monoclonal Antibodies In High Salt Medium In The Presence of Butyrate
TL;DR: Hybridomas were hyper-stimulated to produce industrial-like concentrations of Ig levels by suppressing cell growth and increasing culture longevity by adaptation to higher osmolarity medium and addition of sodium butyrate, and could be used to enhance Mab production of low secreting human hybridomas and immortalised B-cells.
Intracellular responses of productive hybridomas subjected to high osmotic pressure.
TL;DR: It has previously been found hybridoma cells undr hyuerosmotic stress produce higher amounts of antibody, but Sucrose and KCl were found to induce similar trends, except to different extents.