Feng Zhang
Broad Institute
69 Papers
425 Citations
Feng Zhang is an academic researcher from Broad Institute. The author has contributed to research in topics: CRISPR & Biology. The author has an hindex of 14, co-authored 69 publications. Previous affiliations of Feng Zhang include Stanford University & Rutgers University.
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Papers
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening
Julia Joung,Silvana Konermann,Jonathan S. Gootenberg,Omar O. Abudayyeh,Randall Jeffrey Platt,Mark D. Brigham,Mark D. Brigham,Mark D. Brigham,Neville E. Sanjana,Feng Zhang +9 more
TL;DR: In this article, a protocol for genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system is described, where custom or ready-made guide RNA libraries are constructed and packaged into lentiviral vectors for delivery into cells for screening.
1.1K
Massively-parallel single nucleus RNA-seq with DroNc-seq
Naomi Habib,Naomi Habib,Inbal Avraham-Davidi,Anindita Basu,Tyler Burks,Karthik Shekhar,Matan Hofree,Sourav Choudhury,Sourav Choudhury,François Aguet,Ellen Gelfand,Kristin Ardlie,David A. Weitz,Orit Rozenblatt-Rosen,Feng Zhang,Feng Zhang,Aviv Regev,Aviv Regev +17 more
TL;DR: In this paper, a massively parallel single-nucleus RNA sequencing (sNuc-seq) with droplet technology is proposed. But it does not provide high throughput, and it is not suitable for high-dimensional data.
1K
Ongoing global and regional adaptive evolution of SARS-CoV-2.
Nash D. Rochman,Yuri I. Wolf,Guilhem Faure,Pascal Mutz,Feng Zhang,Feng Zhang,Eugene V. Koonin +6 more
TL;DR: In this paper, the authors analyzed more than 300,000 high-quality genome sequences of SARS-CoV-2 variants available as of January 2021, and found that the ongoing evolution of CoV2 during the COVID-19 pandemic is characterized primarily by purifying selection, but a small set of sites appear to evolve under positive selection.
228
Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.
TL;DR: Genomic locus proteomics (GLoPro) is developed, in which CRISPR-based genome targeting, proximity labeling, and quantitative proteomics are combined to discover proteins associated with a specific genomic locus in native cellular contexts.
158
Patent
Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells
Feng Zhang,Matthias Heidenreich,Lukasz Swiech +2 more
- 11 Jun 2014
TL;DR: In this article, the authors provide delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences, including delivery systems and tissues or organ which include post mitotic cells which are targeted as sites for delivery.
149