Fei Liu
Yale University
10 Papers
7 Citations
Fei Liu is an academic researcher from Yale University. The author has contributed to research in topics: RNA & Pseudoknot. The author has an hindex of 7, co-authored 10 publications. Previous affiliations of Fei Liu include University at Albany, SUNY & Howard Hughes Medical Institute.
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Papers
HOTAIR Forms an Intricate and Modular Secondary Structure
Srinivas Somarowthu,Michal Legiewicz,Isabel Chillón,Marco Marcia,Fei Liu,Anna Marie Pyle,Anna Marie Pyle +6 more
TL;DR: The HOTAIR structure reveals a degree of structural organization comparable to well-folded RNAs, like the group II intron, rRNA, or lncRNA steroid receptor activator, and is composed of four independently folding modules, two of which correspond to predicted protein-binding domains.
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Visualizing the secondary and tertiary architectural domains of lncRNA RepA.
TL;DR: This work determined a complete phylogenetically validated secondary-structural map of RepA through SHAPE and DMS chemical probing of a homogeneously folded RNA in vitro and combined UV-cross-linking experiments with RNA modeling methods to produce a three-dimensional model ofRepA functional domains demonstrating that tertiary architecture exists within lncRNA molecules and occurs within specific functional modules.
Native Purification and Analysis of Long RNAs.
Isabel Chillón,Marco Marcia,Michal Legiewicz,Fei Liu,Srinivas Somarowthu,Anna Marie Pyle,Anna Marie Pyle +6 more
TL;DR: How to study lnc RNA global compaction in the presence of divalent ions at equilibrium using sedimentation velocity analytical ultracentrifugation and analytical size-exclusion chromatography as well as how to use these uniform RNA species to determine robust lncRNA secondary structure maps by chemical probing techniques like selective 2'-hydroxyl acylation analyzed by primer extension and dimethyl sulfate probing.
The internal dynamics of mini c TAR DNA probed by electron paramagnetic resonance of nitroxide spin-labels at the lower stem, the loop, and the bulge.
Yan Sun,Ziwei Zhang,Vladimir M. Grigoryants,William K. Myers,Fei Liu,Keith Earle,Jack H. Freed,Charles P. Scholes +7 more
TL;DR: Electron paramagnetic resonance (EPR) at 236.6 and 9.5 GHz probed the tumbling of nitroxide spin probes in the lower stem, in the upper loop, and near the bulge of mini c TAR DNA, finding the slowing of nanosecond motions of large segments of the oligonucleotide was enhanced by increasing the ratio of the nucleocapsid protein NCp7 to mini cTAR DNA from 0 to 2.
104 Exploring the architecture of lncRNA RepA, a key player in X-chromosome inactivation
Fei Liu,Anna Marie Pyle +1 more
TL;DR: folding of a 2-base-pair RNA kissing complex William Stephenson*, Ashely Colvin, Alan A. X. Chen and Pan T.X. Li.
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