Fei He
University of Copenhagen
8 Papers
18 Citations
Fei He is an academic researcher from University of Copenhagen. The author has contributed to research in topics: CRISPR & Mutant. The author has an hindex of 5, co-authored 6 publications.
Chat about Author
Papers
Anti-CRISPR proteins encoded by archaeal lytic viruses inhibit subtype I-D immunity.
Fei He,Yuvaraj Bhoobalan-Chitty,Lan B. Van,Anders L. Kjeldsen,Matteo Dedola,Kira S. Makarova,Eugene V. Koonin,Ditlev E. Brodersen,Xu Peng +8 more
TL;DR: It is demonstrated that AcrID1 protein inhibits the CRISPR–Cas subtype I-D system by interacting directly with Cas10d protein, which is required for the interference stage, and belongs to a conserved family of compact, dimeric αβ-sandwich proteins characterized by extreme pH and temperature stability and a tendency to form protein fibres.
First experimental evidence for the presence of a CRISPR toxin in sulfolobus.
Fei He,Lanming Chen,Xu Peng +2 more
TL;DR: This work provides the first experimental evidence for the presence of a toxin gene in the type I-A CRISPR system of hyperthermophilic archaeon Sulfolobus and demonstrates that the derepression of csa5 and the subsequent Csa5 oligomerization take place in native virus-host systems.
17
Analysis of the Taxonomy, Synteny, and Virulence Factors for Soft Rot Pathogen Pectobacterium aroidearum in Amorphophallus konjac Using Comparative Genomics
TL;DR: The complete genome sequences of 11 bacterial isolates that can cause typical soft rot symptoms in konjac were reported and showed that the pangenome of P. aroidearum is open and that accessory genes are enriched in replication, recombination, and repair.
5
Publisher Correction: Anti-CRISPR proteins encoded by archaeal lytic viruses inhibit subtype I-D immunity.
Fei He,Yuvaraj Bhoobalan-Chitty,Lan B. Van,Anders L. Kjeldsen,Matteo Dedola,Kira S. Makarova,Eugene V. Koonin,Ditlev E. Brodersen,Xu Peng +8 more
TL;DR: In the original version of this Article, molecular weight markers in Figs 1c, 2c,d and 4d were displaced during the production process, so that they were not correctly aligned with the corresponding bands.
Anti-CRISPR-Based and CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2.
David Mayo-Muñoz,Fei He,Jacob Bruun Jørgensen,Poul Kári Madsen,Yuvaraj Bhoobalan-Chitty,Xu Peng +5 more
TL;DR: Two different genome editing approaches to mutate the Sulfolobus islandicus rod-shaped virus 2 (SIRV2) using the archaeon Sulfolia islandicus LAL14/1 and its derivatives as hosts are described and the generated viral mutants are useful for future functional studies including searching for new Acrs.