Fan You
Jiangnan University
35 Papers
72 Citations
Fan You is an academic researcher from Jiangnan University. The author has contributed to research in topics: Fermentation & Pullulanase. The author has an hindex of 8, co-authored 35 publications.
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Papers
Significantly improving the yield of recombinant proteins in Bacillus subtilis by a novel powerful mutagenesis tool (ARTP): Alkaline α-amylase as a case study.
Yingfang Ma,Haiquan Yang,Xianzhong Chen,Bo Sun,Guocheng Du,Zhemin Zhou,Song Jiangning,Fan You,Wei Shen +8 more
TL;DR: This study is the first to report a novel powerful mutagenesis tool (ARTP) that significantly improves the yield of recombinant proteins in B. subtilis and may therefore play an important role in the high expression level of proteins in recombinant microbial hosts.
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Engineering Eschericha coli for Enhanced Tyrosol Production.
TL;DR: This investigation suggests that microbial tyrosol production has application potential, and the reintroduction of the phenylpyruvate decarboxylase gene ARO10 and the aromatic amino acid aminotransferase geneARO8 were introduced into Escherichia coli to generate two recombinant tyosol producers.
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Display of phytase on the cell surface of Saccharomyces cerevisiae to degrade phytate phosphorus and improve bioethanol production
TL;DR: The strain reported here represents a useful novel engineering platform for developing an environment-friendly system for bioethanol production from a corn substrate and could improve ethanol production performance and effectively reduce the discharge of phosphorus.
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Development of an efficient genetic manipulation strategy for sequential gene disruption and expression of different heterologous GFP genes in Candida tropicalis
Lihua Zhang,Xianzhong Chen,Chen Zhen,Zezheng Wang,Shan Jiang,Li Li,Markus Pötter,Wei Shen,Fan You +8 more
TL;DR: A simple and reliable method to improve C. tropicalis by genetic manipulation by using an auxotrophic mutant host defective in orotidine monophosphate decarboxylase (URA3) and a codon-optimized green fluorescent protein-encoding gene (GFP) was functionally expressed.
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Overproduction, purification, and property analysis of an extracellular recombinant fructosyltransferase
TL;DR: The recombinant FruSG had the highest activity after induction for 144 h in a 5-L fermenter, and produced a high yield of FOS, indicating that the enzyme was Mg2+ and Fe2+ dependent.
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