Electrochemical aptasensors for cancer diagnosis in biological fluids – a review

The invention discloses a method for manufacturing microfluidic dielectrophoresis chips through the screen printing technology The method includes the following steps that first, a microelectrode pattern and a microchannel pattern are drawn, and a microelectrode screen printing plate and a microchannel screen printing plate are manufactured; second, through the microelectrode screen printing plate, conductive ink is printed on printed material, and a microelectrode is manufactured; third, through the microchannel screen printing plate, insulating ink capable of solidification is printed on the printed material (obtained in the second step) on which the microelectrode is printed with a target alignment method, and a microchannel is manufactured; fourth, the portion above the microchannel is covered with a sealing-connection cover sheet and solidified so that the sealing-connection cover sheet can be connected with the microchannel in a sealed mode The method effectively solves the problems that a traditional microfluidic dielectrophoresis chip manufacturing method is long in manufacturing period, low in efficiency and not suitable for industrial production on a large scale

Method for manufacturing microfluidic dielectrophoresis chips through screen printing technology

The invention provides preparation and application of an electrochemical micro-fluidic device for nucleic acid isothermal amplification. A micro-fluidic electrochemical sensor device is directly established on a printed electrode. The device comprises two layers of PDMS chip. The micro-channels on the upper layer are used to fully mix various samples. The channel area in the lower layer is divided into a reaction area and a detection area. A temperature-control element is integrated to the device. The electrode surface is subjected to functional modification. Corresponding primers aiming at the hepatitis B virus nucleic acid are designed. Electrochemical reporter molecules are grafted onto the primers. The labeled primers and the nucleic acid to be detected are evenly mixed, then the mixture is injected into a micro-fluidic chip, and a proper temperature is controlled to carry out isothermal amplification reactions on the nucleic acid sample. The labeled primers are combined to the electrode surface through the base pairing effect during the amplification process. Then the virus can be detected through the detection on the reporter molecules. The provided device has the advantages of miniaturization, low cost, and portability, can perform rapid and high-sensitive detection, and is convenient for diagnosis in outdoors/home.

Preparation and application of electrochemical micro-fluidic device for nucleic acid isothermal amplification

The invention provides a preparation method of an electrochemical adapter sensor for detecting fumonisin B1 (FB1). The preparation method comprises the following steps of step one, preparation of an silk-printed carbon electrode (SPCE); step two, preparation of an activated FB1 aptamer solution; step three, preparation of an Au nanoparticles (AuNPs) modified SPCE; step four, preparation of an FB1 aptamer modified AuNPs-SPCE; step five, sealing of spare active sites on the surface of the electrode. According to the preparation method of the electrochemical adapter sensor for detecting the FB1, provided by the invention, the AuNPs are adopted for modifying the surface of the electrode, on one hand, the large amount of AuNPs are loaded on the surface of the electrode, so that a signal amplification function on label-free impedance method detection is obtained; on the other hand, more sulfhydrylated adapters are fixed to the surface of the electrode by utilizing Au-S bonds. An FB1 impedance detection method provided by the invention has the characteristics that the operation is more simple, convenient and flexible, instruments and equipment are simpler, the dosage of a reagent is less, the detection cost is low, and the like.

Preparation method of electrochemical adapter sensor for detecting fumonisin B1 ( FB1)

The invention discloses a centrifuge tube, a detection system and a detection method. The centrifuge tube comprises a tube body, an electrochemical sensor is fit to the inside wall of the tube body, and comprises a reference electrode, a counter electrode and at least one work electrode, first, second and third lead-out electrodes are arranged separately in the outside wall of the tube body, and are electrically connected with the reference, counter and work electrodes respectively, and the work electrodes are in one to one correspondence with the second lead-out electrodes. The detection system comprises a detector and the centrifuge tube, the detector is used to process an electric signal from the electrochemical sensor, place a to-be-detected object in the centrifuge tube, and output ananalysis result according to an electric signal of the electrochemical sensor. Operation is simple.

Centrifuge tube, detection system and detection method

The invention provides an integrated type micro-fluid control electrochemical biological sensing system for rapid biochemical analysis, and an application method of the system. The system comprises a continuous feeding unit, a micro-fluid control chip and a power system, wherein the continuous feeding unit is used for sequentially conveying a forerunner eluant, a sample solution, a sample eluant, a signal probe solution, a signal probe eluant and an electrochemical detection buffer solution; the micro-fluid control chip consists of one or more micro-channel networks; the micro-fluid control chip covers an electrode array so as to form a channel system; capture probes which have interaction with the sample solution are fixed on the upper surface of the electrode array; the channel system is connected with the continuous feeding unit; the power system is used for providing power for the continuous feeding unit. Three techniques of the planar electrode array, the micro-fluid control chip and the continuous feeding unit are innovatively combined together, and the integrated type micro-fluid control electrochemical biological sensing system which is small in size and low in cost and has a wide application prospect is provided.

Integrated type micro-fluid control electrochemical biological sensing system for rapid biochemical analysis and application method of system

The present invention provides an integrated type microfluidic electrochemical biosensor system for rapid biochemical analysis and the usage of the system The system comprising: a continuous feeding unit for sequentially conveying lead eluent, sample solution, sample eluent, signal probe solution, signal probe eluent and electrochemical detection buffer solution; a microfluidic chip consists of one or more micro-channel network, the microfluidic chip covers the electrode array to form a channel system, capture probes which have interaction with the said sample solution fixed on the surface of the electrode array, said channel system is connected with the continuous feed unit; and a power system for providing power to said continuous feeding unit The invention innovatively combine three technologies of planar electrode arrays, microfluidic chip technology and continuous feeding unit together, and the integrated type microfluidic electrochemical biosensing system which is small in size and low in cost and has a wide application prospect is provided

Integrated type microfluidic electrochemical biosensor system and method for rapid biochemical analysis

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Integrated type micro-fluid control electrochemical biological sensing system for rapid biochemical analysis and application method of system

Integrated type microfluidic electrochemical biosensor system and method for rapid biochemical analysis