A helical mycoplasma,Spiroplasma mirum strain SMCA, produced malignant transformation in mouse NIH 3T3 cells and monkey kidney CV-1 cells. The transformed cells exhibited morphological changes consistent with the transformed phenotype, grew in soft agar and produced tumors in athymic and BALB/c mice. Transmission electron microscopy revealed structures morphologically similar to mycoplasmas present in the cytoplasm of transformed but not untransformed 3T cells. The time of inoculation ofS. mirum SMCA to 3T3 cells and the passage level of 3T3 cells affected transformation.

Malignant transformation of NIH-3T3 and CV-1 cells by a helical mycoplasma, Spiroplasma mirum, strain SMCA.

7 – spiroplasma pathogenicity in vivo and in vitro

Summary Spiroplasma mirum grew to high titres, 108 colour-changing units per ml of supernatant medium, and produced cytopathology which consisted of vacuolization, granulation and polynucleation. S. mirum did not grow in cell culture medium (Dulbecco's MEM+10% fœtal bovine serum), thereby indicating the need for cultured cells or a cell culture product. Growth was also obtained from cell-free supernatants from AG-4676 cultures. S. mirum propagated in AG-4676 produced cataracts and death in suckling Wistar rats.

Rabbit lens cell cultures in the characterization of Spiroplasma mirum pathogenicity

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Rabbit lens cell cultures in the characterization of Spiroplasma mirum pathogenicity