Cell-based biosensors and their application in biomedicine.

https://www.mdpi.com/2079-6374/2/2/127/pdf

Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.

PurposeTo improve orthopedic implant ingrowth, knowledge of the effect of chemical surface modifications on vital cell function in vitro is of importance. Early in our investigations we recognized ...

Time-Dependent Metabolic Activity and Adhesion of Human Osteoblast-Like Cells on Sensor Chips with a Plasma Polymer Nanolayer:

Physical Mechanisms during fs Laser Ablation of Thin SiO2 Films

Measurement Instrumentation and Sensors Handbook Electromagnetic, Optical, Radiation, Chemical, and Biomedical Measurement, by J.G. Webster and H. Eren: Scope: reference, handbook. Level: non-specialists, undergraduate, advanced undergraduate, postgraduate, early career researcher, teacher, scientist, engineers

A thin film layer system consisting of platinum (Pt) as conductive layer on a glass substrate and tantalum pentoxide as isolating layer on top of the platinum is attractive for designing biocompatible conductor paths and contact pads for bio sensor chips. For the flexible and rapid patterning of the conductive and the isolating layers, both, the complete removal and the selective ablation of the individual thin films were investigated using ultra-short laser pulses with about 10 ps pulse duration and 1064 nm wavelength at low laser fluences. A platinum film covered with tantalum pentoxide shows a significantly lower ablation threshold than a single Pt film on glass alone when illuminated from the front side. Furthermore, we explored that the tantalum pentoxide film can be removed by glass side illumination from the Pt film, without affecting the Pt film and leaving the Pt film on the glass substrate intact. Those ablation phenomena occur at laser fluences of about 0.2 J/cm2, far below the evaporation limi...

Picosecond laser structuring of thin film platinum layers covered with tantalum pentoxide isolation

In research, pharmacological drug-screening and medical diagnostics, the trend towards the utilization of functional assays using living cells persists. As they are very sensitive, cells are used as signal transducers. For this purpose our research group has developed an automated high-content online measurement system, which is described in this paper. The system is comprised of a pipetting robot sensors for measurement of pH, pO2, electric signals and cell adhesion, a digital microscope and a climate chamber. The robot is used to supply the cells with nutrient solution or active agents that are to be tested, while the sensors monitor the acidification of the medium, the uptake of oxygen and the adhesion of the cells to the substrate. Reflected light as well as fluorescence imaging during the course of an experiment are possible due to the included microscope. Cells and sensors are arranged in a special 24-well micro plate that is placed at a fixed position within the system. Oxygen and pH values are usually measured every 5 to 15 seconds. The running software also allows planning of experiments over a long period of time (several days), which makes the system suitable for applications like pharmaceutical drug screening, in vitro chemo-sensitivity assays, toxicological studies and analytical histopathology.

Automated multi-parametric label free 24 channel real-time screening system

Sensor chips to measure electrical signals of living cells are patterned with lasers from thin films of platinum as the conductive layer and tantalum pentoxide as the isolating layer. The selective
ablations of the transparent tantalum pentoxide alone as well as the complete removal of both layers were investigated
using picosecond laser pulses at different fluences. Ablation threshold values were measured for the irradiation either
from the layer (front) side or from the glass (back) side. We observed complete and selective laser ablation of the films at
low laser fluences.

Rapid prototyping of biocompatible sensor chips by picoseconds laser structuring of a platinum/ tantalum pentoxide thin film layer system

Living cells are extremely complex microsystems, which internally use biochemical and electronic signals for signal processing. These nonlinear and kinetic reactions are conceived as a quantitative collaboration of the cell as a biological system, whose output signals can be indicators for growth, mitosis, morphology, but could also be a quantity for the vitality of the surrounding micro environment on equal terms. These assays based on whole cell analysis techniques are very sensitive when exposed to external influences. The effect of different substances and toxic agents can be observed marker-free and in real time. The potential to understand the dynamics of the cellular processes justified the development of a handheld lab on chip system (see Fig. 1). The cells are regarded here as signal converters. The presented work herein is concerned with the conceptual development of a Handheld device, that have been optimized for the use in mobile biomedical multi-parametric analytics. On the electrochemical basis of sensor chips on a ceramic substrate made in thin-film technology, the measurement of cellphysiological parameters in the extracellular surrounding became possible. Sensors for pH, oxygen partial pressure, impedance, electrical potentials and temperature for the simultaneous in-vitro measurement of metabolic, morphologic and electrophysiological parameters were combined with a miniaturized mixed signal electronic, to allow local (Point of Care) analyses. A focus was put on the simulation of the various circuit parts, as well as the measurement of the electrical impedance. On this basis it was possible for the first time that food analysis could be shown without complex laboratory-technical investigation. The presented measurement results prove that this system approach with the acquired concepts is a serious alternative to commercially available solutions.

Space saving Mixed Signal FPGAs for improving processing power and memory capacity as a replacement for μCs in portable biosensor devices

Zell-Chipsysteme - Mikrosensorarrays für die biologische Grundlagenforschung und Diagnostik

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