Aims: The aims of the present study were to determine VTEC prevalence in manure, slurryand sewage sludge in France and to characterize the VTEC strains isolated (virulence genes and serotype). Methods and Results: Seven hundred and fifty-two samples from 55 farmyard manures, 136 bovine and porcine faeces, 114 slurries, 10 composts, and 437 samples from outflows of sewagewastewater treatment plants were analysed. Twenty-four percent contained isolates which were PCR positive for stx gene. Twenty-one VTEC strains were recovered from positive samples by colony hybridization: 76% of them were positive for stx2 gene, 33% for stx1 gene,and 19% for eae gene. One strain belonged to serotype O157:H7 and two others to serogroups O26 and O55, respectively. Conclusions: Some of the VTEC strains isolated from environments in France should be considered as potentially pathogenic for humans. Significance and Impact of the Study: Appropriate handling or use of manure, slurry andsewage sludge is necessary so that contamination of the environment and food by VTEC can be prevented.

https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2672.2002.01706.x

Prevalence of verotoxin-producing Escherichia coli (VTEC) in slurry, farmyard manure and sewage sludge in France.

Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx1, and 24 were positive for stx2 (10 were positive for stx2vh-a or stx2vh-b, 19 were positive for stx2d, and 15 were positive for stx2e). One strain belonged to serotype O157:H7, and the others belonged to serogroups O2, O8, O11, O26, O76, O103, O113, O121, O141, O166, and O174. The environment is a reservoir in which new clones of STEC that are pathogenic for humans can emerge.

Serotyping, stx 2 Subtyping, and Characterization of the Locus of Enterocyte Effacement Island of Shiga Toxin-Producing Escherichia coli and E. coli O157:H7 Strains Isolated from the Environment in France

Isolation and characterization of shiga toxin-producing Escherichia coli from meat and dairy products

Effects of slaughter processes on pig carcass contamination by verotoxin-producing Escherichia coli and E. coli O157:H7.

Aims: To determination the prevalence of VTEC in pork products and the surrounding environment of the pork plant (slaughterhouse and cutting plant), and characterization of the VTEC strains isolated (virulence genes and serotype).



Methods and Results:  Among the 2146 carcass and pork samples and 876 environmental samples (swabs of surfaces or materials), 328 (15%) and 170 (19%) were PCR-positive for stx genes respectively. VTEC strains were recovered from positive samples by colony hybridization or immunoconcentration, serotyped and genetically characterized. Strains of E. coli O157:H7 were not isolated from 3 uidA-positive samples detected by PCR. The VTEC isolates did not harbour eae, ehx and uidA genes.



Conclusions:  Pigs and pork meat may contain VTEC strains but characterization of the strains based on virulence factors showed that the potential danger of pork meat appears to be low since although all strains harboured a stx gene, they did not have other virulence genes.



Significance and Impact of the Study: General hygiene measures appear to be sufficient and specific hygiene measures for VTEC are not necessary at this time. The porcine VTEC strains isolated in our study probably do not present a hazard.

Prevalence of verotoxin‐producing Escherichia coli (VTEC) and E. coli O157:H7 in French pork

Bien que la plupart des souches de E. coli appartiennent normalement a la flore microbienne intestinale de l'homme et de l'animal, plusieurs groupes distincts possedent des facteurs de pathogenicite specifiques et sont capables de causer des infections intestinales. Ces souches pathogenes se divisent en six groupes differents de E. coli causant des diarrhees par divers mecanismes de virulence: les E. coli enterotoxinogenes (ETEC), les E. coli enteroinvasifs (EIEC), les E. coli enterohemorragiques (EHEC), les E. coli enteropathogenes (EPEC), les E. coli enteroagregants (EAggEC), et les E. coli a adherence diffuse (DAEC). La pathogenicite de la plupart des souches de E. coli, responsables de diarrhees est associee a la presence de plasmides codant une toxine, l'invasivite, et/ou de genes d'adhesion. Les methodes de detection proposees sont analysees et discutees, aussi bien phenotypique, immunologique ou genotypique comme la detection des genes.

Recherche de Escherichia coli pathogènes dans les selles

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Recherche de Escherichia coli pathogènes dans les selles