Ewfw Alton
National Institutes of Health
32 Papers
386 Citations
Ewfw Alton is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Cystic fibrosis & Genetic transfer. The author has an hindex of 17, co-authored 32 publications.
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Papers
The effect of mucolytic agents on gene transfer across a CF sputum barrier in vitro
M Stern,Natasha J. Caplen,JE Browning,Uta Griesenbach,F. Sorgi,Leaf Huang,Dieter C. Gruenert,C Marriot,Ronald G. Crystal,Duncan M. Geddes,Ewfw Alton +10 more
TL;DR: Pre-treatment of CF airways with rhdnase may be appropriate before liposome or adenovirus-mediated gene therapy, and transfection efficiency remained more than 50% of control at the highest concentration.
126
Mucus altering agents as adjuncts for nonviral gene transfer to airway epithelium.
Stefano Ferrari,Chris Kitson,Raymond Farley,R Steel,Christopher Marriott,D A Parkins,Maurizio Scarpa,Brandon J. Wainwright,Martin J. Evans,William H. Colledge,Duncan M. Geddes,Ewfw Alton +11 more
TL;DR: In vivo application of either Nacystelyn or the anticholinergic glycopyrrolate, both clinically used agents, resulted in increased reporter gene expression in the mouse lung, but no significant correction of the bioelectric defect in CF null mice.
126
Using magnetic forces to enhance non-viral gene transfer to airway epithelium in vivo.
Stefania Xenariou,Uta Griesenbach,Stefano Ferrari,P Dean,Ronald K. Scheule,S H Cheng,Duncan M. Geddes,Christian Plank,Ewfw Alton +8 more
TL;DR: Whereas exposure to a magnetic field improved in vitro transfection efficiency, translation to the in vivo setting remains difficult.
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Safety of a single aerosol administration of escalating doses of the cationic lipid GL-67/DOPE/DMPE-PEG5000 formulation to the lungs of normal volunteers.
Sharon Chadwick,HD Kingston,M Stern,RM Cook,BJ O’Connor,M Lukasson,RP Balfour,M Rosenberg,S H Cheng,Alan E. Smith,D. P. Meeker,Duncan M. Geddes,Ewfw Alton +12 more
TL;DR: It is suggested that a single application of aerosol formulation of GL-67A does not result in clinically detectable changes when given by nebulisation into the lungs of normal volunteers and provides an indication of a lipid dose tolerated in man.
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Anti-inflammatory gene therapy directed at the airway epithelium.
Uta Griesenbach,P. Scheid,Elizabeth Hillery,R de Martin,Leaf Huang,Duncan M. Geddes,Ewfw Alton +6 more
TL;DR: Decoy oligonucleotides may be a promising approach for reduction of NFκB-mediated pulmonary inflammation in cystic fibrosis.
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