Eva M. Klein
7 Papers
46 Citations
Eva M. Klein is an academic researcher. The author has contributed to research in topics: Endoplasmic reticulum & Secretory protein. The author has an hindex of 3, co-authored 5 publications.
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Papers
Plant endoplasmin supports the protein secretory pathway and has a role in proliferating tissues.
Eva M. Klein,Laura Mascheroni,Andrea Pompa,Laura Ragni,Thilo Weimar,Kathryn S. Lilley,Paul Dupree,Alessandro Vitale +7 more
TL;DR: It is concluded that the UPR acts differently, at least in part, on the expression of the two chaperones, and an important role for endoplasmin in proliferating plant tissues is indicated.
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Protein domains involved in assembly in the endoplasmic reticulum promote vacuolar delivery when fused to secretory GFP, indicating a protein quality control pathway for degradation in the plant vacuole.
Ombretta Foresti,Francesca De Marchis,Maddalena de Virgilio,Eva M. Klein,Sergio Arcioni,Michele Bellucci,Alessandro Vitale +6 more
TL;DR: Results show that defective secretory proteins with permanently exposed sequences normally involved in oligomerization can be delivered to the vacuole by secretory traffic, which strongly suggests the existence of a plant vacuolar sorting mechanism devoted to the disposal of defectivesecretory proteins.
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Expression of CLAVATA3 fusions indicates rapid intracellular processing and a role of ERAD.
Francesca De Marchis,Sara Colanero,Eva M. Klein,Davide Mainieri,Viviana M. Prota,Michele Bellucci,Giampiero Pagliuca,Elisa Zironi,Teresa Gazzotti,Alessandro Vitale,Andrea Pompa +10 more
TL;DR: The results indicate that processing of CLV3 starts intracellularly in an early compartment of the secretory pathway and that ERAD could play a regulatory or direct role in the active peptide synthesis.
8
Two γ-zeins induce the unfolded protein response.
Lorenzo Brocca,Melania Zuccaro,Giovanna Frugis,Davide Mainieri,Claudia Adriana Marrano,Laura Ragni,Eva M. Klein,Alessandro Vitale,Emanuela Pedrazzini +8 more
TL;DR: This paper showed that 16-kDa γ-zein significantly activates the INOSITOL REQUIRING ENZYME1/BASIC LEUCINE ZIPPER 60 (bZIP60) UPR branch but not the bZIP28 branch or autophagy-leading to induction of major UPR-controlled genes that encode folding helpers that function inside the ER.
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Aptamer loaded superparamagnetic beads for selective capturing and gentle release of activated protein C
Nasim Shahidi Hamedani,Felix Lucian Happich,Eva M. Klein,H. Rühl,Günter Mayer,Johannes Oldenburg,Jens Müller,Bernd Pötzsch +7 more
TL;DR: In this article , the authors presented a one-step activation of APC from a commercial source of protein C (PC, Ceprotin) followed by rapid and efficient purification using an APC-specific aptamer, HS02-52G, loaded on MyOne superparamagnetic beads.