Eva Frei
German Cancer Research Center
10 Papers
495 Citations
Eva Frei is an academic researcher from German Cancer Research Center. The author has contributed to research in topics: DNA & Topoisomerase. The author has an hindex of 10, co-authored 10 publications.
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Papers
The anticancer agent ellipticine on activation by cytochrome P450 forms covalent DNA adducts.
TL;DR: The DNA adduct formation described is a novel mechanism for the ellipticine action and might in part explain its tumor specificity.
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DNA adduct formation by the anticancer drug ellipticine in rats determined by 32P postlabeling.
Marie Stiborová,Andrea Breuer,Dagmar Aimová,Martina Stiborova-Rupertova,Manfred Wiessler,Eva Frei +5 more
TL;DR: The results presented here are the first report showing the formation of CYP‐mediated covalent DNA adducts by ellipticine in vivo and confirm the formation in rat tissues in vivo as a new mode of ellipticines action.
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Rat microsomes activating the anticancer drug ellipticine to species covalently binding to deoxyguanosine in DNA are a suitable model mimicking ellipticine bioactivation in humans.
TL;DR: Rats are more suitable models than rabbits mimicking the metabolic activation of ellipticine in humans for the formation of covalent DNA adducts, and deoxyguanosine is identified as the target for P450-mediated ellipticines binding to DNA using polydeoxyribonucleotides and de oxygenguanoine 3'-monophosphate.
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Mammalian peroxidases activate anticancer drug ellipticine to intermediates forming deoxyguanosine adducts in DNA identical to those found in vivo and generated from 12-hydroxyellipticine and 13-hydroxyellipticine.
TL;DR: It is shown that ellipticine binds covalently to DNA after its activation by peroxidases, and species, 2 carbenium ions, ellipticin‐13‐ylium and ellipticines‐12‐ylia, are proposed as reactive species generating 2 major DNA adducts seen in vivo in rats treated with elliptICine.
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Covalent binding of the anticancer drug ellipticine to DNA in V79 cells transfected with human cytochrome P450 enzymes
TL;DR: The results presented here are the first report showing the formation of CYP-mediated covalent DNA adducts by ellipticine in cells in culture, and confirm the formation in cells as a new mechanism of ellipticines action.
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