Eugeny Volkov
Moscow State University
5 Papers
52 Citations
Eugeny Volkov is an academic researcher from Moscow State University. The author has contributed to research in topics: Oligonucleotide & Restriction enzyme. The author has an hindex of 3, co-authored 5 publications.
Chat about Author
Papers
Oligonucleotide‐peptide conjugates as potential antisense agents
Eugeny M. Zubin,Elena A. Romanova,Eugeny Volkov,Vadim N. Tashlitsky,Galina A. Korshunova,Zoe A. Shabarova,Tatiana S. Oretskaya +6 more
TL;DR: A novel approach of fragment coupling of pre‐synthesized peptides to the 2′‐position of a selected nucleotide within an otherwise protected oligonucleotide chain attached to a solid support is developed.
37
Detection of Abasic Sites in DNA by Electrochemical, Immunoelectrochemical and Acoustic Methods Using OsO4, 2,2′-bipyridine as a Probe for Unpaired Thymine Residues
Martin Bartošík,Viktor Gajdoš,Pavel Kostečka,Miroslav Fojta,Emil Paleček,Eugeny Volkov,Tatiana S. Oretskaya,Tibor Hianik +7 more
TL;DR: In this article, a comparative analysis of the detection of DNA damage modeled by presence of abasic sites (AP) at defined positions using the chemical modification of the DNA by the complex of osmium tetroxide-2,2-bipyridine (Os, bipy) that selectively binds to unpaired thymine residues in the damaged DNA.
15
Detection of glycosylase, endonuclease and methyltransferase enzyme activities using immobilized oligonucleotides.
Anna Sudina,Eugeny Volkov,Tatiana S. Oretskaya,Nikolai Naryshkin,M. G. Ivanovskaya,Elena A. Kubareva +5 more
TL;DR: The assay will most valuable in the applications where rapid estimation of enzyme activity is necessary and use of fluorescent labeling facilitates detection while use of solid phase‐immobilized substrates facilitates product separation, improved assay sensitivity, and increases throughput of assay.
4
Controlling the enzymatic activity of a restriction enzyme by light.
Benno Schierling,Ann-Josée Noël,Wolfgang Wende,Le Thi Hien,Eugeny Volkov,Elena A. Kubareva,Tatiana S. Oretskaya,Michael Kokkinidis,Andreas Römpp,Bernhard Spengler,Alfred Pingoud +10 more
TL;DR: Modulating the activity of a restriction enzyme with light by cross-linking two suitably located cysteine residues with a bifunctional azobenzene derivative, which can adopt a cis- or trans-configuration when illuminated by UV or blue light, respectively, enzymatic activity can be controlled in a reversible manner.
A Convenient Solid-Phase Method for the Synthesis of Novel Oligonucleotide-Folate Conjugates
Eugenia V Kazanova,Eugeny M. Zubin,Anna V. Kachalova,Eugeny Volkov,Tatiana S. Oretskaya,Dmitry A. Stetsenko,Marina Gottikh +6 more
TL;DR: The preparation of two batches of a polymer support for the incorporation of folic acid into oligonucleotides is described, which permits the regioselective attachment of a target nucleic acid sequence through its 3′-end to either the α-or γ-carboxyl group of L-glutamic acid, respectively.