Elizabeth A. Boydston

TL;DR: The highly efficient targeting of three genes in human pluripotent cells using zinc-finger nuclease (ZFN)–mediated genome editing is reported, demonstrating that ZFNs can be used to generate reporter cells by targeting non-expressed genes in hESCs and hiPSCs.

TL;DR: It is demonstrated that easily-generated linear donors with extremely short homology regions drive transgene integration into 5–10% of chromosomes and that oligonucleotide donors with single-stranded 5′ overhangs complementary to those made by ZFNs are efficiently ligated in vivo to the DSB.

TL;DR: Localized translation of peroxisomal membrane proteins is crucial for cellular functions as discussed by the authors , but it is difficult to find the optimal protein translation strategy for each cell in real-world applications.

TL;DR: In this article , the authors use CRISPR-based screens to functionally profile the entire genome of the model apicomplexan parasite Toxoplasma gondii during mouse infection, and uncover over 300 genes that modulate parasite fitness in mice with previously unknown roles in infection.