Elisabeth Memmel
University of Würzburg
10 Papers
11 Citations
Elisabeth Memmel is an academic researcher from University of Würzburg. The author has contributed to research in topics: Chemistry & Click chemistry. The author has an hindex of 6, co-authored 10 publications.
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Papers
Super-Resolution Imaging of Plasma Membrane Glycans†
Sebastian Letschert,Antonia Göhler,Christian Franke,Nadja Bertleff-Zieschang,Elisabeth Memmel,Sören Doose,Jürgen Seibel,Markus Sauer +7 more
TL;DR: These results demonstrate a homogeneous distribution of N-acetylmannosamine (ManNAc)-, N- acetylgalactosamines (GalNAc-, and O-linked N -acetylglucosamine-modified plasma membrane proteins in different cell lines with densities of several million glycans on each cell surface.
92
Biocompatible Azide-Alkyne "Click" Reactions for Surface Decoration of Glyco-Engineered Cells.
TL;DR: A systematic study elucidates the design space for the cytotoxic effects of the copper catalyst on NIH 3T3 fibroblasts and on HEK 293‐F cells and details effective and biocompatible conditions for CuAAC‐based modification of glyco‐engineered cells in comparison to its copper free alternative.
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Metabolic glycoengineering of Staphylococcus aureus reduces its adherence to human T24 bladder carcinoma cells
TL;DR: In this article, metabolic glycoengineering with N-azidoacetyl-glucosamine (GlcNAz) has been successfully applied to S. aureus for the first time.
27
Synthesis and application of water-soluble, photoswitchable cyanine dyes for bioorthogonal labeling of cell-surface carbohydrates.
TL;DR: Alkyne functionalized dyes were used for bioorthogonal click reactions by labeling of metabolically incorporated sugar-azides on the surface of living neuroblastoma cells, which were applied to direct stochastic optical reconstruction microscopy (dSTORM) for the visualization of cell-surface glycans in the nm-range.
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Investigating infection processes with a workflow from organic chemistry to biophysics: the combination of metabolic glycoengineering, super-resolution fluorescence imaging and proteomics.
Jürgen Seibel,Simone König,Antonia Göhler,Sören Doose,Elisabeth Memmel,Nadja Bertleff,Markus Sauer +6 more
TL;DR: The use of special synthesized carbohydrate labels, in combination with new super-resolution imaging approaches, allows access to both mapping and identification of cell surface glycoproteins well below the diffraction limit, and will clarify which surface molecules are involved in bacterial adherence with potential implications for bacterial and viral infection prevention.
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