Eleanor J Brant
University of Florida
5 Papers
2 Citations
Eleanor J Brant is an academic researcher from University of Florida. The author has contributed to research in topics: Genome editing & Biology. The author has an hindex of 2, co-authored 5 publications. Previous affiliations of Eleanor J Brant include Montana State University.
Chat about Author
Papers
CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement.
TL;DR: A comprehensive review of CRISPR/Cas development can be found in this paper, where the advantages and disadvantages of the technology, recent applications, and future considerations are discussed as well as the major challenges for wider application.
CRISPR/Cas9 mediated targeted mutagenesis of LIGULELESS-1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle.
TL;DR: In this article, the authors described CRISPR/Cas9 mediated targeted mutagenesis of the liguleless-1 (LG1) in sorghum and phenotypic changes in mono-and bi-allelic lg1 mutants.
22
Efficient genome editing in wheat using Cas9 and Cpf1 (AsCpf1 and LbCpf1) nucleases
Abstract: Genome editing can be used to create new wheat varieties with enhanced performance. Clustered regularly interspaced short palindromic repeat (CRISPR) is a powerful tool for knockout generation, precise modification, multiplex engineering, and the activation and repression of target genes. Targeted mutagenesis via RNA-guided genome editing using type II CRISPR-Cas9 is highly efficient in some plant species, but not in others. One possible solution is to use newly discovered variants of genome editing enzymes such as the class 2 system component Cpf1 (CRISPR from Prevotella and Francisella 1) in place of the more commonly used Cas9. We compared the editing efficiency of Cas9 and two Cpf1 orthologs, AsCpf1 (Acidaminococcus spp. BV3L6) and LbCpf1 (Lachnospiraceae bacterium ND2006) in wheat (Triticum aestivum). LbCpf1 had a higher editing efficiency for the target gene TaPDS than AsCpf1 and Cas9, and Cas9 induced more off-target mutations than AsCpf1 and LbCpf1, suggesting that CRISPR-LbCpf1 is a powerful genome editing tool for polyploid plants such as wheat.
21
CRISPR/Cas-mediated genome editing in sorghum — recent progress, challenges and prospects
TL;DR: In this paper, the authors describe the progress in genome editing of sorghum and review the remaining challenges and prospects of emerging gene editing technologies for rapid precision breeding of this crop, which is a versatile crop with great potential as a sustainable food, feed, and bioenergy source.
6
Plant small non-coding RNAs and their roles in biotic stresses.
Eleanor J Brant,Hikmet Budak +1 more
TL;DR: The current knowledge of the biogenesis of the primary sRNA classes, microRNA (miRNA) and small nuclear RNA (snRNA), and their respective secondary classes are reviewed, and the roles of sRNAs in plant–pathogen interactions are discussed.