Care during freeze-drying of bovine pericardium tissue to be used as a biomaterial: a comparative study.

Inhibition of calcification of bovine pericardium after treatment with biopolymers, E-beam irradiation and in vitro endothelization

Bovine pericardium (BP) is an extensively used biomaterial utilised in a wide range of biomedical devices such as bioprosthetic heart valves. However, the mechanical testing techniques that assess soft biomaterial tissue like BP are varied with no common method utilised across the literature, producing variations and contradictions in reported values. Uniaxial testing is a common technique used to measure traditional mechanical characteristics of ultimate tensile strength (UTS), modulus and the percentage strain at failure. The aim of this study was to take two standard uniaxial test parameters, strain rate and the number of preconditioning cycles and to elucidate recommendations for the standardisation of a uniaxial method, while also measuring not so common parameters of low modulus and hysteresis. Samples post uniaxial testing were treated and analysed with scanning electron microscopy (SEM) and an imaging software (ImageJ) to measure the effect of the parameters on the crimping structure and orientation of the collagen fibres. The study recommends an extension rate of 10 mm/min and 5 preconditioning load-unload cycles as a starting point for the standardisation of a uniaxial testing method. The image analysis of the collagen structure carried out provides a quantitative assessment of the BP post mechanical testing and allows for a better understanding of the behaviour of BP under stress.

Assessment of the uniaxial experimental parameters utilised for the mechanical testing of bovine pericardium

Exposure to ionizing radiation is unavoidable for noncancerous cells during the external radiotherapy process. Increasing the dose delivery fraction times leads to increasing the endothelial cell damage. Vascular abnormalities are commonly associated with the alternation of endothelium biomechanical properties. The goal of the present study was to quantify the elastic and viscoelastic properties of human umbilical vein endothelial cells (HUVECs) using the micropipette aspiration technique in conjunction with a theoretical model while an 8 Gy dose was given in four fractions. Confocal imaging was performed for evaluation of cytoskeletal changes during fractionation 60Co radiotherapy. The results indicated an increase in elastic modulus from 29.87 ± 1.04 Pa to 46.69 ± 1.17 Pa while the fractional doses increased from 0 Gy to 8 Gy along with the obvious cytoskeletal changes. Moreover, in the creep behavior of radiated groups, a significant decrease was shown in the time constant and viscoelastic properties. On the other hand, it was observed that the change in the biomechanical properties of the cells while applying a single fraction of 8 Gy was not exactly the same as that in the properties of the radiation-exposed cells while delivering an 8 Gy dose at 2 Gy per fraction. The observed differences in the biomechanical behavior of endothelium provide a quantitative description of radiobiological effects for evaluating the dose-response relationship as a biological dosimetry procedure.

Dose-dependent 60 Co γ-radiation Effects on Human Endothelial Cell Mechanical Properties

Atomic force microscopy and mechanical testing of bovine pericardium irradiated to radiotherapy doses

The pericardium is an anistropic composite material made up of collagen and elastin fibres embedded in an amorphous matrix mainly composed of proteoglycan and hyaluronan. The collagen fibres are arranged in layers, with different directions of alignment in each layer, giving rise to interesting mechanical properties of pericardium, including the ability to undergo large deformation during performance of regular physiological functions. The present study aims to investigate the effect of penetrating photon ionising radiation on bovine pericardium tissue, being part of a study of the effect of cardiac doses received in breast radiotherapy and the possibility that this can give rise to cardiovascular complications. Irradiation doses in the range 5–80 Gy were used. To characterise the various mechanical properties [elastic modulus, stress relaxation, ultimate tensile strength (UTS) and fracture] a uniaxial tensile test method was applied. The preliminary results reflect the wide inter-sample variations that are expected in dealing with tissues, with only a weak indication of increase in the UTS of the pericardium tissue with increase in radiation dose. Such an effect has also been observed by others, with reduction in UTS at doses of 80 Gy.

Effect of penetrating ionising radiation on the mechanical properties of pericardium

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

E. Woods

Author Tools

Chat about Author

Papers

Effect of penetrating ionising radiation on the mechanical properties of pericardium

Atomic force microscopy and mechanical testing of bovine pericardium irradiated to radiotherapy doses