Hydrophilic interaction chromatography (HILIC) in the analysis of antibiotics.

The principles and applications of fluorescence detection and fluorescence introducing reagents and methods in HPLC are reviewed. The design and requirements for fluorescence detectors, flow cells and excitation sources and the conversion of non-fluorescent compounds into fluorescent products by pre-column and post-column derivatization reactions are discussed. For the applications the emphasis is on drug analysis, where possible in biological fluids (serum, urine, etc.). The last paragraphs are divided in a number of sections in which newly developed and some scarcely used reagents are mentioned shortly; a more complete treatment is given of the reagents and labels most frequently used in the derivatization of certain functional groups. In this discussion the methods of derivatization as well as the selectivity, stability, fluorescence behaviour of the reagents/labels and derivatives and the reaction conditions are included. An up-to-date survey of the applications of fluorescence detection in l...

Fluorescence Detection in High Performance Liquid Chromatography

An HPLC method has been developed for the determination of the cephalosporin antibiotic Ro 13-9904 in plasma, urine, and bile of dogs and of human volunteers using the technique of ion-pair chromatography with a LiChrosorb RP-18 column. The three mobile phases employed contained tetrapentyl-, tetraoctyl- and hexadecyltrimethyl-ammonium bromide, respectively, as lipophilic counterions. The chromatographic conditions chosen allowed simple and rapid sample preparation. Plasma was deproteinized with ethanol and the supernatant was directly injected onto the column; urine and bile were diluted with mobile phase and injected without any purification. The detection limit for the cephalosporin was about 0.5 μg/ml for plasma samples and approximately 5 μg/ml for bile and urine.

Determination of the cephalosporin Ro 13‐99041 in plasma, urine, and bile by means of ion‐pair reversed phase chromatography

This review covers the fundamental developments in the field of column liquid chromatography (LC) during the period of 1984-1985. In an attempt to conserve space, patents and theses have been excluded. Only articles published in English, French, German, and Russian with some exceptions have been included. As compared to the previous review (Anal. Chem. 1984, 56, 300R-340R), all of the tables dealing with selected applications have been deleted and sections on automation and data handling, ion chromatography, trace analysis and sample preparation, and novel applications have been included. New sections that have been added are hydrophobic interaction chromatography, micellar mobile phases, fast LC, and indirect photometric direction. This review is not a comprehensive coverage of all LC literature. The attempt has been made to critically select only those references that reflect fundamental developments in LC theory, methodology, and instrumentation. The main data base for this review was CA Selects (HPLC and GPC) from October 31, 1983, to December 2, 1985. This represented over 8000 abstracts of which 1200 were used. In addition, each author used other search routines to augment coverage from CA Selects. 195 references.

Column liquid chromatography

Determination of cefixime in biological samples by reversed-phase high-performance liquid chromatography.

Quantitative liquid chromatographic determination of cefatrizine in serum and urine by fluorescence detection after post-column derivatization

Separation of some cephalosporin derivatives by ion-pair reversed-phase high-performance liquid chromatography.

Determination of cefatrizine in serum and urine by reversed-phase high-performance liquid chromatography.

A thin-layer chromatographic procedure for the quantitative determination of cephalexin in cephradine formulations is described The thin-layer chromatography is carried out on a silica gel layer and the quantitation is by UV scanning of the chromatogram The procedure was transferred to high-performance liquid chromatography on a microparticulate silica gel column with a similar eluent system The cephalexin content of a few commercial cephradine samples is determined by using these techniques The results of these procedures are compared to those obtained with high-performance liquid chromatography on a C 18 reverse phase column

Application of thin layer and high performance liquid chromatography to the separation and determination of cephalexin in cephradine, in bulk powder and in pharmaceuticals

The behaviour of some macrolide antibiotics in high-performance liquid chromatography on silica gel using an isohydric eluent system and its modifications are described. These modifications involve water content and diethylamine content variations in the eluent, and also column temperature variations, using column packing material previously described. Extention to other types of silica gel packing materials was also performed. The experiments showed excellent results for the separations of the macrolide antibiotic complexes Turimycins and Spiramycins and proved that the chromatographic process is not only an adsorption one but that partitioning effects also play an important role.

Applications of a Modified “Isohydric Solvent System” in HPLC on Silica Gel for the Analysis of the Macrolide Antibiotics Turimycins and Spiramycins

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