A method for detection of capripoxvirus(CaPV)through the loop-mediated isothermal amplification(LAMP)was established.The conditions for amplification of CaPV DNAs were optimized using LAMP Real Time Turbidimeter LA-320.It was found that at 62℃for 60min,highly efficient amplification of the goat pox virus(GPV),sheep pox virus(SPV)and bovine lump skin disease virus(LSDV)were achieved.The minimum detection of viral nucleic acid content was up to 3.1×10-2 pg/μL,104 times higher than the PCR method recommended by OIE.The method was rapid,specific and easy to operate.

Development of a Loop-mediated Isothermal Amplification Method for Rapid Detection of Capripoxvirus

i Acknowledgements ii Declaration iii List of figures viii List of tables xii Chapter

Development of DNA-based assays for the detection of Batrachochytrium dendrobatidis in environmental samples and amphibians

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Development of DNA-based assays for the detection of Batrachochytrium dendrobatidis in environmental samples and amphibians