Dongye Wang
Rutgers University
5 Papers
21 Citations
Dongye Wang is an academic researcher from Rutgers University. The author has contributed to research in topics: RNA polymerase & Gene expression. The author has an hindex of 4, co-authored 5 publications. Previous affiliations of Dongye Wang include Howard Hughes Medical Institute.
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Papers
Structural Basis of Transcription Inhibition by Fidaxomicin (Lipiarmycin A3)
Wei Lin,Kalyan Das,David Degen,Abhishek Mazumder,Diego Duchi,Dongye Wang,Yon W. Ebright,Richard Y. Ebright,Elena V. Sineva,Matthew Gigliotti,Aashish Srivastava,Sukhendu Mandal,Yi Jiang,Yu Liu,Ruiheng Yin,Zhening Zhang,Edward T. Eng,Dennis Thomas,Stefano Donadio,Haibo Zhang,Changsheng Zhang,Achillefs N. Kapanidis,Richard H. Ebright +22 more
TL;DR: A cryo-EM structure of Mycobacterium tuberculosis RNAP holoenzyme in complex with Lpm at 3.5-Å resolution shows that Lpm binds at the base of the RNAP "clamp."
104
Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Staudinger-Bertozzi ligation
Anirban Chakraborty,Abhishek Mazumder,Miaoxin Lin,Adam Hasemeyer,Qumiao Xu,Dongye Wang,Yon W. Ebright,Richard H. Ebright +7 more
TL;DR: A three-step procedure comprising unnatural-amino-acid mutagenesis with 4-azido-phenylalanine, Staudinger-Bertozzi ligation with a probe-phosphine derivative, and in vitro reconstitution of RNAP enables the efficient site-specific incorporation of a fluorescent probe, a spin label, a cross-linking agent, a cleaving agent, an affinity tag, or any other biochemical or biophysical probe, at any site of interest
14
Patent
Bipartite inhibitors of bacterial RNA polymerase
Richard H. Ebright,Dongye Wang +1 more
- 04 Nov 2006
TL;DR: In this article, the authors proposed a structural formula X-α-Y, where X is an moiety that binds to the Rif pocket of a bacterial RNA polymerase, Y is a moiety which binds to a secondary channel of a BPR, and α is a linker.
5
Structural basis of transcription inhibition by fidaxomicin (lipiarmycin A3)
Wei Lin,Kalyan Das,David Degen,Abhishek Mazumder,Diego Duchi,Dongye Wang,Yon W. Ebright,Richard Y. Ebright,Elena V. Sineva,Matthew Gigliotti,Aashish Srivastava,Sukhendu Mandal,Yi Jiang,Yu Liu,Ruiheng Yin,Zhening Zhang,Edward T. Eng,Dennis Thomas,Stefano Donadio,Haibo Zhang,Changsheng Zhang,Achillefs N. Kapanidis,Richard H. Ebright +22 more
TL;DR: It is proposed that Lpm inhibits transcription initiation by trapping an open-clamp conformational state, thereby preventing simultaneous engagement of transcription initiation factor σ regions 2 and 4 with promoter -10 and -35 elements.
3
Azide-specific labeling of biomolecules by Staudinger-Bertozzi ligation phosphine derivatives of fluorescent probes suitable for single-molecule fluorescence spectroscopy.
Anirban Chakraborty,Dongye Wang,Dongye Wang,Yon W. Ebright,Yon W. Ebright,Richard H. Ebright,Richard H. Ebright +6 more
TL;DR: The synthesis of phosphine derivatives of three fluorescent probes that have a brightness and photostability suitable for single-molecule fluorescence spectroscopy and microscopy: Alexa488, Cy3B, and Alexa647 are described.