Ding Kai
Shanghai Jiao Tong University
12 Papers
29 Citations
Ding Kai is an academic researcher from Shanghai Jiao Tong University. The author has contributed to research in topics: Intein & Antibody. The author has an hindex of 5, co-authored 12 publications.
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Papers
Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein
TL;DR: It has been demonstrated that different TGE batches are reproducible from lot to lot in overall cell growth, product yield, and product qualities including isoelectric point, binding affinity, secondary structure, and thermal stability.
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Producing defucosylated antibodies with enhanced in vitro antibody-dependent cellular cytotoxicity via FUT8 knockout CHO-S cells
Huifang Zong,Lei Han,Ding Kai,Jiaxian Wang,Tao Sun,Xinyu Zhang,Cedric Cagliero,Hua Jiang,Yueqing Xie,Jianrong Xu,Baohong Zhang,Jianwei Zhu +11 more
TL;DR: The results demonstrated the potential of using the clustered regularly interspaced short palindromic repeats‐CRISPR associated nuclease 9 technology in cell line engineering for biopharmaceutical industrial applications.
22
Enhanced production of anti-PD1 antibody in CHO cells through transient co-transfection with anti-apoptotic genes Bcl-x L and Mcl-1
Xinyu Zhang,Lei Han,Huifang Zong,Ding Kai,Yuan Yuan,Jingyi Bai,Yuexian Zhou,Baohong Zhang,Jianwei Zhu +8 more
TL;DR: This work provides an effective way to increase viability of host cells through delaying apoptosis onset, thus, raise production yield of biopharmaceuticals without the process of generating stable cell lines and subsequent screening.
22
Naturally split intein Npu DnaE mediated rapid generation of bispecific IgG antibodies
Lei Han,Huifang Zong,Yuexian Zhou,Zhidi Pan,Jie Chen,Ding Kai,Yueqing Xie,Hua Jiang,Baohong Zhang,Huili Lu,John Gilly,Jianwei Zhu +11 more
TL;DR: A platform called Bispecific Antibody by Protein Trans-Splicing (BAPTS) to synthesize BsAbs with natural human IgG structure and no chain mispairing is reported.
19
Patent
Establishment and application of CHO cell line for producing fucose-free monoclonal antibody
Jianwei Zhu,Huifang Zong,Baohong Zhang,Hua Jiang,Yueqing Xie,Han Lei,Ding Kai,Tao Sun +7 more
- 24 May 2017
TL;DR: In this paper, a CRISPR/Cas9-based method was used to encode an FUT8 gene of a CHO cell to obtain a fucose-free monoclonal antibody.
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