Diana M. Downs
University of Georgia
133 Papers
1.8K Citations
Diana M. Downs is an academic researcher from University of Georgia. The author has contributed to research in topics: Thiamine & Salmonella enterica. The author has an hindex of 36, co-authored 133 publications. Previous affiliations of Diana M. Downs include University of Wisconsin-Madison & Cornell University.
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Papers
Thiamin biosynthesis in prokaryotes.
Tadhg P. Begley,Diana M. Downs,Steven E. Ealick,Fred W. McLafferty,Van Loon Ap,Sean V. Taylor,Nino Campobasso,Hsiu-Ju Chiu,Cynthia Kinsland,Jason J. Reddick,Jun Xi +10 more
TL;DR: The X-ray structures of thiamin phosphate synthase and 5-hydroxyethyl-4-methylthiazole kinase have been completed and the genes coding for the thienin transport system (thiBPQ) have been identified.
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Complex Metabolic Phenotypes Caused by a Mutation in yjgF, Encoding a Member of the Highly Conserved YER057c/YjgF Family of Proteins
TL;DR: This work represents the first detailed phenotypic characterization of yjgF mutants in any organism and provides important clues as to the function of this highly conserved class of proteins.
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ThiC Is an [Fe-S] Cluster Protein That Requires AdoMet To Generate the 4-Amino-5-hydroxymethyl-2-methylpyrimidine Moiety in Thiamin Synthesis
TL;DR: The data showed this protein generated HMP when AIR, S-adenosylmethionine (AdoMet), and an appropriate reducing agent were present, and it was shown that ThiC carries an oxygen labile [Fe-S] cluster essential for this activity.
Characterization of thiL, Encoding Thiamin-monophosphate Kinase, in Salmonella typhimurium
Eric A. Webb,Diana M. Downs +1 more
TL;DR: It is demonstrated that altered thiamin-monophosphate kinase activity resulted in decreased repression of transcription ofThiL, consistent with a dual role for ThiL inde novo biosynthesis and in salvage of exogenous thiam in bacteria.
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Metabolic Defects Caused by Mutations in the isc Gene Cluster in Salmonella enterica Serovar Typhimurium: Implications for Thiamine Synthesis
Elizabeth Skovran,Diana M. Downs +1 more
TL;DR: Analysis of the metabolic consequences of two insertions in the isc gene cluster of Salmonella enterica serovar Typhimurium showed that IscS was required for the synthesis of nicotinic acid and the thiazole moiety of thiamine and that one or more additionalIsc gene products were required for a distinct step in theThiazole biosynthetic pathway.
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