Dhruv Sud
University of Michigan
16 Papers
164 Citations
Dhruv Sud is an academic researcher from University of Michigan. The author has contributed to research in topics: Fluorescence-lifetime imaging microscopy & Cytotoxic T cell. The author has an hindex of 9, co-authored 16 publications.
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Papers
Quantitative measurement and control of oxygen levels in microfluidic poly(dimethylsiloxane) bioreactors during cell culture.
Geeta Mehta,Khamir Mehta,Dhruv Sud,Jonathan W. Song,Tommaso F. Bersano-Begey,Nobuyuki Futai,Yun Seok Heo,Mary Ann Mycek,Jennifer J. Linderman,Shuichi Takayama +9 more
TL;DR: The data indicate that despite oxygen diffusion through PDMS, uptake of oxygen by cells inside the perfused PDMS microchannels induces an axial oxygen concentration gradient, with lower levels recorded in downstream regions.
Fluorescence lifetime imaging microscopy.
TL;DR: Various applications of FLIM, ranging from commonly used fluorescence resonance energy transfer (FRET)-FLIM to fluorescence correlation spectroscopy, MS-MP-, and video-rate FLIM are also outlined in this chapter.
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Optical imaging in microfluidic bioreactors enables oxygen monitoring for continuous cell culture
TL;DR: The fluorescence lifetime-based imaging approach described avoids intensity-based artifacts (including photobleaching and concentration variations) and provides a technique with high spatial discrimination for oxygen monitoring in continuous cell culture systems.
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Time-resolved optical imaging provides a molecular snapshot of altered metabolic function in living human cancer cell models
TL;DR: Starkly higher intracellular oxygen and NADH levels in living SEG-1 vs. HET-1 cells were detected by FLIM and attributed to altered metabolic pathways in malignant cells.
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Fluorescence Lifetime Imaging Microscopy for Quantitative Biological Imaging
TL;DR: This chapter begins by introducing the basic theory of fluorescence lifetime, including the characteristics of fluorophore decay, followed by a discussion of factors affecting fluorescence lifetimes and the potential advantages offluorescence lifetime as a source of image contrast.
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