Devin L. Trudeau
Weizmann Institute of Science
14 Papers
8 Citations
Devin L. Trudeau is an academic researcher from Weizmann Institute of Science. The author has contributed to research in topics: Cellulase & Thermostability. The author has an hindex of 10, co-authored 14 publications. Previous affiliations of Devin L. Trudeau include Mayo Clinic & California Institute of Technology.
Chat about Author
Papers
Automated Design of Efficient and Functionally Diverse Enzyme Repertoires
Olga Khersonsky,Rosalie Lipsh,Ziv Avizemer,Yacov Ashani,Moshe Goldsmith,Haim Leader,Orly Dym,Shelly Rogotner,Devin L. Trudeau,Jaime Prilusky,Pep Amengual-Rigo,Victor Guallar,Dan S. Tawfik,Sarel J. Fleishman +13 more
TL;DR: FuncLib, an automated method for designing multipoint mutations at enzyme active sites using phylogenetic analysis and Rosetta design calculations, is described and opened the way to designing highly efficient and diverse catalytic repertoires.
231
On the potential origins of the high stability of reconstructed ancestral proteins
TL;DR: It is found that inferred ancestors of the serum paraoxonase (PON) enzyme family, including the mammalian ancestor, exhibit dramatically increased thermostabilities compared with the extant, human enzyme (up to 30 °C higher melting temperature); two potential origins of this unexpectedly high stability are discussed.
Protein engineers turned evolutionists-the quest for the optimal starting point.
Devin L. Trudeau,Dan S. Tawfik +1 more
TL;DR: Recent developments in both disciplines with respect to how one can identify the best starting points for directed evolution are outlined, such that highly efficient and robust tailor-made enzymes can be obtained with minimal optimization.
113
Design and in vitro realization of carbon-conserving photorespiration
Devin L. Trudeau,Christian Edlich-Muth,Jan Zarzycki,Marieke Scheffen,Moshe Goldsmith,Olga Khersonsky,Ziv Avizemer,Sarel J. Fleishman,Charles A. R. Cotton,Tobias J. Erb,Dan S. Tawfik,Arren Bar-Even +11 more
TL;DR: A kinetic–stoichiometric model indicates that the identified synthetic shunts could potentially enhance carbon fixation rate across the physiological range of irradiation and CO2, even if most of their enzymes operate at a tenth of Rubisco’s maximal carboxylation activity.
113
Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures.
TL;DR: The creation of the most stable reported fungal endoglucanase, a derivative of Hypocrea jecorina (anamorph Trichoderma reesei) Cel5A, is described by combining stabilizing mutations identified using consensus design, chimera studies, and structure‐based computational methods.